Method for selectively breeding Honglian type rice blast-resistant sterile lines

A technology for rice blast resistance and rice blast resistance gene is applied in the field of breeding red lotus type rice blast resistance sterile lines, which can solve problems such as poor ventilation conditions and excessive rice growth, achieve restoration of farmland ecology, reduce input and use, Achieving the effect of increasing agricultural efficiency

Inactive Publication Date: 2013-08-28
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, farmers often increase the amount of fertilizer and water, and apply nitrogen fertilizer in the later stage, resulting in excessive growth of rice, lush branches and leaves, and poor ventilation conditions, which will encourage the occurrence of rice blast

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Collect the rice blast resistant rice material RGD-7S (including Pi1 and Pi2 resistant to blast disease) of Honglian CMS Luohong 3A, maintainer Luohong 3B, and known molecular markers (molecular markers RM224 and AP5930). Gene).

[0023] (2) In March 2009, Luohong 3B was used as the female parent and RGD-7 was used as the male parent for artificial crossing during earing in Lingshui, Hainan, and the seeds of Luohong 3B and F1 were collected.

[0024] (3) The Luohong 3B and F1 collected in step (2) were planted in Wuhan, Hubei in May 2009. When heading, the F1 was used as the female parent, and the Luohong 3B was used as the male parent for backcrossing, and Luohong was collected. 3B and BC1F1 seeds.

[0025] (4) In November 2009, BC1F1 and Luohong 3B collected in step (3) were planted in Lingshui, Hainan. After transplanting into the field, the BC1F1 plants were identified by molecular markers and identified by indoor inoculation of rice blast to screen for plants ...

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Abstract

The invention discloses a method for selectively breeding Honglian type rice blast-resistant sterile lines, belonging to the technical field of crop breeding. The method for selectively breeding Honglian type rice blast-resistant sterile lines disclosed by the invention comprises the following steps of: collecting Honglian type sterile lines, maintainer lines and rice blast-resistant rice materials, and combining the conventional hybridization and backcross breeding technology of Honglian type sterile maintainer lines and rice blast-resistant rice materials with the auxiliary selection technology marked by rice blast molecules, specifically: firstly, breeding the maintainer lines into rice blast-resistant maintainer lines, and then, carrying out multi-generation backcross with the sterile lines to selectively breed Honglian type sterile lines having rice blast-resistant characteristics. The method for selectively breeding Honglian type rice blast-resistant sterile lines disclosed by the invention has the advantages of being clear in target, precise and high-efficiency in technology and free from ecological and food safety risks and public concerns of transgenic rice, and is capable of reducing investment and use of pesticide, realizing agricultural efficiency and farmer income and providing support for restoring farmland ecology.

Description

[0001] technical field [0002] The invention belongs to the technical field of crop breeding, and relates to a method for breeding red lotus-type rice blast-resistant sterile lines. Background technique [0003] Rice blast, a fungal venereal disease, is one of the important diseases of rice, which can cause a substantial reduction in yield, in severe cases, a 40-50% reduction in yield, or even no harvest. It occurs in every rice area in the world, and it is also one of the important contents of rice disease research. There are many kinds of rice blast diseases, most of which occur on the leaves and nodes, and can cause different degrees of yield reduction after occurrence, especially the early and severe occurrence of panicle blast or node blast, which can cause white ears and even extinction. In recent years, the annual occurrence area of ​​rice blast has increased year by year, and local outbreaks are not uncommon, with an average annual disease area of ​​no less tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02C12Q1/18C12R1/645
Inventor 朱仁山朱英国胡骏黄文超刘文军
Owner WUHAN UNIV
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