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Method for inducing in-vitro ovulation of poultry ovarian follicles

A technology for poultry and mature follicles, which is applied to animal cells, vertebrate cells, germ cells, etc., can solve the problems of in vitro culture, maturation and ovulation research of poultry follicles.

Inactive Publication Date: 2013-09-04
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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Problems solved by technology

[0003] At present, the in vitro culture, maturation and ovulation techniques of mammalian follicles are very mature. Due to the large difference in the regulation mechanism of follicle development between mammals and poultry, it is not possible to directly apply the method of mammals to induce in vitro ovulation in poultry.
At present, there are few studies on the in vitro culture, maturation and ovulation of poultry follicles

Method used

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  • Method for inducing in-vitro ovulation of poultry ovarian follicles
  • Method for inducing in-vitro ovulation of poultry ovarian follicles

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example

[0018] 1. Breeding and selection of experimental animals

[0019] During the peak egg production period (25-40 weeks old), the light time is 16 hours, the daily feed intake is 120g, and sufficient clean drinking water is given. Observe the egg-laying interval. Generally, one egg is laid in a little more than 24 hours, and the continuous egg-laying cycle is more than 30 days. In the peak period of laying hens in this state, the maturity time and degree of F1 are easy to grasp.

[0020] 2. Acquisition of F1 follicles

[0021] Select 8 laying hens in the peak period that meet the above conditions, 4 of them were bled to death by jugular vein bleeding within 10 minutes after laying eggs, and 4 were killed by jugular vein bleeding within 30 minutes after laying eggs, and the abdominal feathers were washed with 10% 84 disinfectant Until it is completely wet, place it on a clean bench, and wipe the abdomen feathers with a 75% alcohol swab. Use sterilized surgical scissors to cut o...

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Abstract

The invention provides a method for inducing the in-vitro ovulation of mature poultry ovarian follicles. The invention also provides a preparation method of a culturing medium for inducing the in-vitro ovulation of mature poultry ovarian follicles. The preparation method is characterized in that insulin, transferrin, sodium selenite, penicillin, streptomycin, lutropin, follitropin, progesterone, estrogen and an M199 culture medium are mixed under an aseptic condition to prepare a liquid culture medium. Experiments in the invention prove that the induced ovulation of mature ovarian follicles (F1) separated from the ovary of a laying hen (30min after laying) in a laying peak is realized after the in-vitro culturing through using the culture medium provided by the invention. The gonadotropin and steroid hormone in the culture medium provided by the invention approach the gonadotropin and steroid hormone in the poultry in-vivo normal physiological environment, so the culture medium can be widely applied to researches about the poultry ovarian follicle developmental biology.

Description

technical field [0001] The invention relates to a method for inducing ovulation in vitro of poultry mature follicles, more specifically, the invention relates to a method for preparing a medium for inducing ovulation in poultry in vitro by using gonadotropin and steroid hormones. technical background [0002] The number of laying hens and laying ducks in my country ranks first in the world. The development of follicles in the ovaries of egg-using poultry directly affects egg production performance. By studying the mechanism of poultry follicle development and its influencing factors, it will help to further improve the production performance of poultry for eggs. In addition, compared with mammals, there is no corpus luteum formation on the ovary of birds after ovulation, so follicles can develop, mature, and ovulate continuously, and the granulosa cell layer and theca cell layer of follicles are easy to separate, which is beneficial to the study of different follicles in th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/075
Inventor 冯京海张敏红姜礼文熊艳丹
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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