Culturing medium used for tubercle bacilli, and preparation method thereof

Abstract

A culturing medium used for tubercle bacilli includes 1ml of a standard preparation improved Lowenstein-Jensen culture medium and 10-15mug of inosine, and inosine can directly permeate cell membranes to enter cells, so the cells in a low-energy anoxic stationary state resume normal activities, thereby the culturing period and the treatment period of the tubercle bacilli are shortened.

Description

technical field [0001] The invention relates to a culture medium and a preparation method, in particular to a culture medium and a preparation method for Mycobacterium tuberculosis. Background technique [0002] Tuberculosis is a common disease, and its frequently-occurring disease is a Class B infectious disease that seriously endangers human health. According to the analysis of the results of a sample survey of tuberculosis epidemiology in my country in 2000, the current epidemic situation of tuberculosis in my country is very serious, showing the characteristics of five more than one high, that is, the number of tuberculosis infections More than 400 million people in the country have been infected with tuberculosis; there are many tuberculosis patients in China, and there are 5 million tuberculosis patients in my country, of which 2 million are contagious; the number of tuberculosis deaths is large, and the number of tuberculosis deaths in my country is about 150,000 every ...

AI Technical Summary

Problems solved by technology

At the same time, the isolation and culture of mycobacteria also provides test strains for bacterial species identification and drug sensitivity monitoring. The treatment of tuberculosis after a definite diagnosis is the key to drug selection, and drug sensitivity testing is required to select drugs. Drug sensitivity selection is effective Drugs can not only reduce the burden on patients but also avoid the side effects caused by the use of ineffective drugs. At present, the most classic method of tuberculosis culture and tuberculosis culture + drug sensitivity is to use modified Roche medium for culture, modified Roche medium (Lowensten-Jensen, L-J ) is recommended by the International Union Against Taberculosis and Lung Disease (IUATLD), the culture used and compared with other media, this medium can cultivate tuberculosis from the beginning of culture to the obvious tuberculosis can be seen It takes about 16-56 days for bacterial colonies and 14-60 days for drug sensitivity, so the culture period is longer

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