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Copper ion colorimetry detecting kit and detecting method thereof

A technology for detecting kits and copper ions, which is applied in the field of environmental and biological analysis to achieve the effects of high sensitivity, easy observation, and simple operation

Active Publication Date: 2015-07-01
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the kit for detecting copper ions based on the colorimetric method of copper ion-catalyzed etching of gold nanorods in China.

Method used

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  • Copper ion colorimetry detecting kit and detecting method thereof
  • Copper ion colorimetry detecting kit and detecting method thereof
  • Copper ion colorimetry detecting kit and detecting method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Detection of copper ions in drinking water

[0025] The kit includes hydrobromic acid solution, gold nanorod solution containing cetyl ammonium bromide (CTAB), standard color card C and colorimetric tube;

[0026] The hydrobromic acid preparation: obtained by diluting 38% analytically pure hydrobromic acid, the concentration is 0.5M hydrobromic acid solution.

[0027] Preparation of gold nanorod solution containing cetyl ammonium bromide (CTAB):

[0028] 1) Gold nanorods are obtained as follows:

[0029] Synthesis of gold seed solution: Take 50 μL of chloroauric acid (50 mM) solution into 7.7 mL of 0.1 M cetyl ammonium bromide (CTAB) solution and shake for a few seconds, then add 600 μL of ice-cold sodium borohydride (0.01M) to accelerate Shake for a few minutes, the color will stop when it turns from bright yellow to purple gray, and use it after standing for 2 hours in an environment of 26 degrees Celsius.

[0030] To synthesize nanorods, add chloroauric...

Embodiment 2

[0045] Embodiment 2: detection of copper ions in biological samples

[0046] The kit includes hydrobromic acid solution, gold nanorod solution containing cetyl ammonium bromide (CTAB), standard color card C and colorimetric tube;

[0047]The hydrobromic acid preparation: obtained by diluting 38% analytically pure hydrobromic acid, the concentration is 0.5M hydrobromic acid solution.

[0048] Preparation of gold nanorod solution containing cetyl ammonium bromide (CTAB):

[0049] 1) Gold nanorods are obtained as follows:

[0050] Synthesis of gold seed solution: Take 50 μL of chloroauric acid (50 mM) solution into 7.7 mL of 0.1 M cetyl ammonium bromide (CTAB) solution and shake for a few seconds, then add 600 μL of ice-cold sodium borohydride (0.01M) to accelerate Shake for a few minutes, the color will stop when it turns from bright yellow to purple gray, and use it after standing for 2 hours at 26 degrees Celsius

[0051] To synthesize nanorods, add chloroauric acid (50mM) ...

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Abstract

The invention relates to the technical field of environmental and biological analysis, and in particular relates to a copper ion colorimetry detecting kit and a detecting method thereof. The kit comprises a hydrobromic acid solution, a gold nanorod solution containing cetyl trimethyl ammonium bromide (CTAB), a standard colorimetric card C and a colorimetric tube, wherein the gold nanorod solution containing the CTAB is formed by dissolving gold nanorods in a CTAB solution under the condition that the concentration of the gold nanorods is within 0.5 to 10nM and the concentration of the CTAB is with 0.01 to 100mM; and the concentration of hydrobromic acid in the hydrobromic acid solution is within 0.05 to 5.0M. The kit for detecting copper ions has the advantages of being high in sensitivity, good in selectivity, easy to operate, easy in naked-eye observation and the like. Under the debugging of a systematic experiment condition, the detection on the copper ions in environmental and biological samples can be realized.

Description

technical field [0001] The invention relates to the technical field of environmental and biological analysis, in particular to a copper ion colorimetric detection kit and a detection method thereof. Background technique [0002] Copper ions are one of the most important essential elements in the human body, and it plays an important role in the function of many cellular enzymes and proteins. However, when the accumulation of copper ions in the human body exceeds a certain limit, it will lead to copper metabolism disorder, which will bring great harm to the human body. For example, copper metabolism disorder will increase the probability of suffering from Alzheimer's disease and Parkinson's disease. In the water environment, because copper ions cannot be decomposed, they have bioaccumulation and biomagnification effects in organisms, so that the higher the food chain, the copper ion content in the body is also doubled, which causes The danger is also greater. As humans at t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N21/33
Inventor 陈兆鹏张志阳陈令新
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI