Method for measuring response of anoplophora chinensis to volatile substances, and trapping device

Abstract

The invention discloses a method for measuring for measuring response of anoplophora chinensis to volatile substances. The method comprises the following steps of cutting tentacles of adult anoplophora chinensis along base parts; cutting a small amount of tentacle tips; horizontally putting the cut tentacles on an electrode through a conductive adhesive; introducing every reference sample into gas chromatography; carrying out gas chromatography-electroantennographic detection; repeating the experiments; carefully comparing peak shapes and retention time of compounds on chromatogram of the gas chromatography-electroantennographic detection, so as to determine active substances; further determining the active substances by wild attractive activity experiment of the anoplophora chinensis; and then screening out effective attractive chemical compound monomers according to experiment results. The active substances are obtained by directly detecting extracts of the volatile substances. Compared with previous data, the data obtained by the invention is more reliable and effective. Besides, working efficiency is increased to a large extent.

Description

technical field [0001] The invention belongs to the technical field of star beetle research, and in particular relates to a method for measuring volatile substances of star beetle and a trapping device. Background technique [0002] The star beetle is a kind of long beetle endemic to Japan, China and Korea, and is generally considered a pest by the locals. Each female star beetle can lay up to 200 eggs in a single mating, and each egg is hidden separately in the bark of the tree. When the larva hatches, it chews into the tree, creating a tunnel for pupation. The period from egg laying to pupa and adult can reach 12-18 months. Adults appear from May to July and live in flat to middle-altitude mountainous areas. Adults are occasionally phototaxis at night, and beetle infestation can kill many different hardwood trees. The main biological functions of the adult beetle beetle include feeding to supplement nutrition, completing mating and laying eggs to reproduce offspring. B...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the embodiment of the present invention is to provide a method and trapping device for measuring the volatile substances of the star beetle, aiming to solve the problem of repetitive process, cumbersome operation and trapping the star beetle in the current domestic technology for measuring the behavioral response of the star beetle. Less problem with traps with larger flight capabilities

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