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Preparation method of white, low-fat and odorless silkworm pupa protein

A silkworm chrysalis protein and white technology, which is applied in the preparation method of peptides, chemical instruments and methods, animal/human peptides, etc., can solve problems affecting product quality and nutrient content, and the quality of silkworm chrysalis protein, and simplify the subsequent separation process , improve quality and reduce environmental pollution

Active Publication Date: 2015-01-14
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The production process of silk reeling dry silkworm chrysalis is as follows: fresh silkworm chrysalis passes through fresh chrysalis, air-dried chrysalis (drying, anti-corrosion, about 110°C, 1h), wet chrysalis after boiling cocoons, lining silkworm chrysalis after silk reeling, silkworm chrysalis after mechanical delining and high temperature heating Dried silkworm chrysalis (thoroughly dried, about 140-150°C, 3-4 hours) can leave the factory after several steps, but after the final high-temperature drying treatment, the silkworm chrysalis contained in it is oxidized due to the oxidation of the silkworm chrysalis oil and the adhesion of the silkworm chrysalis skin. The quality of silkworm chrysalis protein has declined, and the current research on silkworm chrysalis protein is carried out by using dried silkworm chrysalis after silk reeling. In view of the above problems, most of the processes are treated with a mixture of alkaline solution, hydrogen peroxide and organic solvents to achieve removal. Odor and decolorization requirements, but this treatment method has the problem that the protein structure is further decomposed, affecting product quality and nutrient content, etc.

Method used

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  • Preparation method of white, low-fat and odorless silkworm pupa protein
  • Preparation method of white, low-fat and odorless silkworm pupa protein

Examples

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Effect test

Embodiment 1

[0035] The preparation method of white low-fat and odorless silkworm chrysalis protein of the present invention comprises the following steps:

[0036] (1) Silkworm chrysalis pretreatment: select the silkworm chrysalis after mechanical delining, remove bad pupae, stiff pupae and other impurities, put them into a pulverizer for crushing, and then put them into a homogenizer, according to the solid-liquid weight ratio of 1:3 Proportionally add a certain amount of water, homogenize and stir at a speed of 350r / min, and stir for 30min to obtain a silkworm chrysalis protein mixture.

[0037] (2) Filtration of silkworm chrysalis skin: filter the silkworm chrysalis protein mixture obtained by homogenization with a plate and frame filter, select a 200-mesh filter cloth, and remove the filter residue, mainly to remove the silkworm chrysalis skin and retain the protein filtrate; the filter residue is processed by a homogenizer again and then filtered , repeated 2 times, and combined filt...

Embodiment 2

[0068] The preparation method of white low-fat and odorless silkworm chrysalis protein of the present invention comprises the following steps:

[0069] (1) Silkworm chrysalis pretreatment: select the silkworm chrysalis after mechanical delining, remove bad pupae, stiff pupae and other impurities, put them into a pulverizer for crushing, and then put them into a homogenizer, according to the solid-liquid weight ratio of 1:2 Proportionally add a certain amount of water, homogenize and stir at a speed of 400r / min, and stir for 45min to obtain a silkworm chrysalis protein mixture.

[0070] (2) Filtration of silkworm chrysalis skin: filter the silkworm chrysalis protein mixture obtained by homogenization with a plate and frame filter, select a 250-mesh filter cloth, and remove the filter residue, mainly to remove the silkworm chrysalis skin and retain the protein filtrate; the filter residue is processed by a homogenizer again and then filtered , repeated 1 to 2 times, and combined...

Embodiment 3

[0077] The preparation method of white low-fat and odorless silkworm chrysalis protein of the present invention comprises the steps:

[0078] (1) Silkworm chrysalis pretreatment: select the silkworm chrysalis after mechanical delining, remove bad pupae, stiff pupae and other impurities, put them into a pulverizer and pulverize them, and then put them into a homogenizer, according to the solid-liquid weight ratio of 1:5 Proportionally add a certain amount of water, homogenize and stir at a speed of 500r / min, and stir for 60min to obtain a silkworm chrysalis protein mixture.

[0079] (2) Filtration of silkworm chrysalis skin: Filter the silkworm chrysalis protein mixture obtained by homogenization with a plate and frame filter, select a filter cloth of 200-250 mesh, and remove the filter residue, mainly to remove the silkworm chrysalis skin and retain the protein filtrate; the filter residue is processed by a homogenizer again Post-filtering was repeated 3 times, and the filtrat...

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Abstract

The invention provides a preparation method of white, low-fat and odorless silkworm pupa protein. The preparation method comprises the following steps of: with mechanically taken-off silkworm pupa as the raw material for production, preprocessing the silkworm pupa; filtering silkworm pupa skin; preparing rough silkworm pupa protein; adding ethanol to preprocess the silkworm pupa protein in a decolorizing and deoiling manner; adding a non-polar solvent and natural zeolite to process the silkworm pupa protein in the decolorizing and deoiling manner again; and then drying and crushing to obtain the finished silkworm pupa protein. According to the preparation method, the conventional method of extracting the raw material of silkworm pupa protein, extracting the silkworm pupa protein firstly and extracting the silkworm pupa oil secondly is changed, and the steps of heating and drying the raw silkworm pupa for extracting the silkworm pupa oil are avoided, so that the energy consumption is reduced, and the cost is reduced; a polar solvent and a non-polar solvent are adopted and used for extracting the silkworm pupa oil, respectively; the odor and the silkworm pupa oil in the silkworm pupa protein can be effectively removed by virtue of the physical absorption and deodorization effects of the natural zeolite, and the quality of the silkworm pupa oil also can be improved by distributive extracting.

Description

technical field [0001] The invention belongs to the technical field of animal protein preparation, and in particular relates to a preparation method of silkworm chrysalis protein. Background technique [0002] Silkworm chrysalis (Silkworm pupa) is the chrysalis of Bombyx mori, a silkworm moth, and is a large by-product of the silk industry. According to the statistics of the Ministry of Commerce of the People's Republic of China, in 2009 the output of dried silkworm cocoons, the raw material of silk, reached 782,100 tons. Half of the dry weight of silkworm cocoons is silkworm chrysalis. The silkworm chrysalis protein accounts for 45%-50% of the dry silkworm chrysalis mass. It has been determined that the silkworm chrysalis protein contains 18 kinds of amino acids needed by the human body. It is an ideal high-quality pure natural full-valent protein. Due to the lack of deep processing technology, a large number of silkworm chrysalis are used as feed and fertilizer, and the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/435C07K1/30
Inventor 赵钟兴廖丹葵谢美萱孙建华童张法
Owner GUANGXI UNIV
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