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Three-dimensional cell co-culture model based on PDMS (polydimethylsiloxane) and preparation method thereof

A three-dimensional cell and co-cultivation technology, applied in tissue cell/virus culture devices, biochemical equipment and methods, biochemical instruments, etc., can solve the problem of not being able to meet the co-culture interaction at the same time, and achieve easy control and repeatability. , the effect of simple process

Inactive Publication Date: 2013-09-18
FIRST AFFILIATED HOSPITAL OF DALIAN MEDICAL UNIV
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although many different cell co-culture methods and three-dimensional cell culture methods are widely used, they are all limited by technical problems and have their own shortcomings and deficiencies. Function and cell three-dimensional growth and movement changes, collecting enough cells for subsequent molecular biology testing and other aspects of experimental needs

Method used

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  • Three-dimensional cell co-culture model based on PDMS (polydimethylsiloxane) and preparation method thereof
  • Three-dimensional cell co-culture model based on PDMS (polydimethylsiloxane) and preparation method thereof
  • Three-dimensional cell co-culture model based on PDMS (polydimethylsiloxane) and preparation method thereof

Examples

Experimental program
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Effect test

experiment example 1

[0034] Experimental example 1: The three-dimensional cell co-culture model based on PDMS is applied to the co-culture of two kinds of cells in the horizontal direction and the interaction between the two is observed.

[0035] Put this model body 1 into the 6-hole plate and press it tightly so that the bottom is close to the bottom of the 6-hole plate, and two cuboid-shaped stoppers 7 are respectively close to the two sides of the opening 8 of the compartment 2 to form a In one space, inject 20ul Matrigel (BD Company, USA) into the space, incubate at 37°C for 1 hour to solidify, and take out the cuboid-shaped stoppers 7 on both sides after the Matrigel solidifies. At this time, two culture pools, namely the right culture pool 4 and the left culture pool 3, are separated by the solidified Matrigel.

[0036] One type of cell is inoculated into the left culture pool 3, and the other type of cell is inoculated into the right culture pool 4. The culture fluid of the two can pass thr...

experiment example 2

[0038] Experimental Example 2: The model of the present invention is applied to the experiment that one kind of cell (or components in the culture medium) affects another kind of cell's three-dimensional shape and movement.

[0039] The model placement and cell inoculation methods are the same as in Experimental Example 1. Inoculate one kind of cell (or a culture solution containing a certain component) into the culture tank on the left side, and inoculate another kind of cell into the culture tank on the right side, you can dynamically observe the effect of the former on the latter, and make the latter move into the Matrigel The influence of growth and migration, so as to observe the changes in the three-dimensional shape and movement of cells. It is suitable for experiments such as cell migration, chemotaxis, invasion, angiogenesis of vascular endothelial cells in vitro, and tumor cell invasion.

[0040] For example, glomerular endothelial cells were inoculated in the right...

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Abstract

The invention discloses a three-dimensional cell co-culture model based on PDMS (polydimethylsiloxane). The three-dimensional cell co-culture model comprises a model body, wherein a left culture pool and a right culture pool are arranged on the inner side of the model body, an interlayer is arranged between the left culture pool and the right culture pool, an opening communicating the left culture pool with the right culture pool is formed in the interlayer, and stoppers jointed with the side face of the interlayer are arranged in the left culture pool and the right culture pool. According to the three-dimensional cell co-culture model based on PDMS, a co-culture system of two kinds of cells is established in the same plane so as to dynamically observe the interaction of the two kinds of cells, cell migration, chemotaxis, invasion and tube formation tests can be carried out, molecular biology detection can be conveniently carried out on the cells in situ or the cells are connected to perform the detection, and in addition, the defect that a vertical co-culture method cannot eliminate gravity interference is overcome; and the three-dimensional cell co-culture model can be repeatedly used and has the advantages of being simple and practicable, good in repeatability, cheap, safe and the like. The invention further discloses a preparation method of the model. The preparation method is simple in technique, liable to operate and easy to control.

Description

technical field [0001] The invention relates to a cell culture model and a preparation method, in particular to a PDMS-based three-dimensional cell co-culture model and a preparation method. Background technique [0002] In vitro cell co-culture (co-culture) is to mix and co-culture two kinds of cells (from the same tissue or from different tissues), so that the shape and function of one of the cells can be stably expressed and maintained for a long time . Cell co-culture technology can simulate the microenvironment generated in vivo, which is convenient for better observing the interaction between cells and cells, and between cells and the culture environment, as well as exploring the mechanism of action and possible targets of drugs, filling the gap between monolayer cell culture and Whole Animal Experimentation Divide. [0003] This technology can mainly involve experiments and applications in the following aspects: 1. Cell-to-cell contact, causing direct contact of pla...

Claims

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Application Information

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IPC IPC(8): C12M3/00
Inventor 林洪丽张团儿滕佳琪
Owner FIRST AFFILIATED HOSPITAL OF DALIAN MEDICAL UNIV
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