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Method for preparing composite enzyme/probiotic preparation for feed by bioconverting sweet potato stem and leaf and sesame cake pulp

A technology of probiotic preparations and sweet potato stems and leaves, applied in animal feed, animal feed, applications, etc., can solve the problems of difficult digestion of monogastric animals, and achieve the effects of low-cost, high-efficiency biotransformation, and strong enzyme production capacity

Inactive Publication Date: 2014-08-20
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, while the cake is rich in protein, it also contains a relatively high amount of plant fiber, which is not easy for monogastric animals to digest.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) Fully mix 10kg of fermented Pu-erh tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of sweet potato stems and leaves, 5kg of sesame cake, 2kg of wheat bran, 1kg of apple pomace, and 22kg of water. After 48 hours of acclimatization and cultivation, a leavening agent is formed;

[0028] 2) Fully mix 20kg of starter in step 1) with 600kg of sweet potato stems and leaves, 300kg of sesame cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm. Naturally ventilated, at 40°C Ferment for 4 days to obtain the compound enzyme and probiotic preparation for feed.

[0029] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 48.23IU / g; total cellulase activity reaches 4.55IU / g; beta-glucosidase activity reaches 4.55IU / g; reached 9.59IU / g; endoglucanase activ...

Embodiment 2

[0031] 1) Fully mix 10kg of fermented Pu-erh tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of sweet potato stems and leaves, 5kg of sesame cake, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water. After 48 hours of acclimatization and cultivation, a leavening agent is formed;

[0032] 2) Fully mix 20kg of starter in step 1) with 600kg of sweet potato stems and leaves, 300kg of sesame cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm. Naturally ventilated, at 40°C Ferment for 4 days to obtain the compound enzyme and probiotic preparation for feed.

[0033] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 47.54IU / g; total cellulase activity reaches 4.62IU / g; beta-glucosidase activity reaches 47.54IU / g; reached 9.22IU / g; endoglucanase acti...

Embodiment 3

[0035] 1) Fully mix 10kg of fermented Pu-erh tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of sweet potato stems and leaves, 5kg of sesame cake, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water. After 36 hours of acclimatization and cultivation, a leavening agent is formed;

[0036] 2) Fully mix 10kg of starter in step 1) with 600kg of sweet potato stems and leaves, 300kg of sesame cake, 60kg of wheat bran, 30kg of apple pomace, and 1000kg of water, and use solid-state fermentation with a material thickness of 10cm, naturally ventilated, and fermented at 35°C After 5 days, a compound enzyme and probiotic preparation for feed was obtained.

[0037] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 47.77IU / g; total cellulase activity reaches 4.34IU / g; beta-glucosidase activity reaches 47.77IU / g; reached 9.76IU / g; endoglu...

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Abstract

The invention discloses a method for preparing a composite enzyme / probiotic preparation for feed by bioconverting sweet potato stem and leaf and sesame cake pulp, which comprises the following steps: thoroughly mixing Pu'er tea after pile fermentation, shiitake stick, agrocybe aegerita stick, first sweet potato stem and leaf, first sesame cake pulp, first wheat bran, first apple pomace and first water, and carrying out acclimatization culture to form a leavening agent; and thoroughly mixing the leavening agent, second sweet potato stem and leaf, second sesame cake pulp, second wheat bran, second apple pomace and second water, and fermenting to obtain the composite enzyme / probiotic preparation for feed. In the invention, the microecologically-stable food-grade safe florae are utilized to ferment the sweet potato stem and leaf and sesame cake pulp to prepare the composite enzyme / probiotic preparation for feed with favorable performance indexes, thereby implementing low-cost high-efficiency bioconversion of the sweet potato stem and leaf and sesame cake pulp. Meanwhile, the method disclosed by the invention has the advantages of easy growth of microorganisms, high enzyme activity and extensive fermentation process, and can easily implement industrialized large-scale production.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for fermenting sweet potato stems and leaves and sesame cakes with micro-ecologically stable food-grade safe flora to prepare a compound enzyme and probiotic preparation for feed. Background technique [0002] my country is a populous country with extremely tight arable land, and there is a huge gap in the demand for feed raw materials, especially energy feed and protein feed. [0003] Lignocellulose is the most abundant and cheapest renewable resource on earth. The production and processing of agricultural products and food also produces a large amount of lignocellulosic by-products. There are many kinds of lignocellulose, such as various crop straws, urban cellulosic waste, agricultural product processing by-products such as corncobs, vegetable stems and leaves, fruit and vegetable residues, etc. The components of lignocellulose mainly include cellulo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/00A23K1/14
Inventor 阮晖纪晓燚伍星主吴渊于骅陈功诸文颖杨璐杜姗姗
Owner ZHEJIANG UNIV
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