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Method for preparing food-grade lutein crystal from marigold extract

A marigold and lutein technology, applied in the direction of organic chemistry, can solve the problems of low product yield, complicated steps, and long time consumption, and achieve the effects of reducing production costs, convenient operation, and cost reduction

Inactive Publication Date: 2013-09-25
INNOBIO CORP LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] In view of the in-depth study of the above problems, the present invention provides a method for preparing food-grade lutein crystal powder from marigold extract to overcome the high cost, complicated steps, long time-consuming and low product yield in the current lutein preparation method And other issues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] 20.0g marigold extract, in which the lutein content is 156.29mg / g, add 95% ethanol whose volume is 3 times the mass of the extract, stir evenly, then add 20% of the mass of the extract as solid sodium hydroxide, at 50°C React for 6 hours until the saponification reaction is complete, add 100mL of tap water to dilute and cool down to 35°C, crystallize under slow stirring for 16 hours, adjust the pH value to 8-9 with glacial acetic acid and filter to obtain filter cake I; add 100mL of 50% ethanol to filter cake I The aqueous solution was washed at 50°C and then filtered to obtain filter cake II; the filter cake II was washed with 60 mL of 95% ethanol and then filtered, and the filter cake was dried at 60°C after adding antioxidants to obtain 3.50 g of lutein crystal powder, of which The UV content of lutein is 83.27%. According to HPLC detection, the content of all-trans lutein is 92.45%, and the pure yield of lutein is 86.20%.

Embodiment 2

[0046] 20.0g marigold extract, wherein the lutein content is 156.29mg / g, add 88% isopropanol whose volume is 5.5 times of the mass of the extract, stir well, then add 30% of the mass of the extract as solid sodium hydroxide, 50 React at ℃ for 3 hours until the saponification reaction is complete, add 105mL of tap water to dilute and cool to 45℃, crystallize under slow stirring for 24 hours, adjust the pH value to 8-9 with glacial acetic acid and filter to obtain filter cake I; add 80mL60 % isopropanol aqueous solution was washed at 50°C and then filtered to obtain filter cake II; the filter cake II was washed with 40 mL of 95% ethanol and then filtered, and the filter cake was dried at 80°C after adding antioxidants. 3.65 g of lutein crystal powder was obtained, of which the UV content of lutein was 81.52%, and the content of all-trans lutein was 91.32% through HPLC detection, and the pure yield of lutein was 86.93%.

Embodiment 3

[0048] 20.0g of marigold extract, in which the lutein content is 153.55mg / g, add 95% ethanol whose volume is 7 times the mass of the extract, stir evenly, then add 35% of the mass of the extract as solid sodium hydroxide, at 50°C React for 5 hours until the saponification reaction is complete, add 170mL of tap water to dilute and lower the temperature to 40°C, crystallize under slow stirring for 24 hours, adjust the pH value to 8-9 with glacial acetic acid and filter to obtain filter cake I; add 100mL of 60% ethanol to filter cake I The aqueous solution was washed at 60°C and then filtered to obtain filter cake II; the filter cake II was washed with 120 mL of 95% ethanol and then filtered, and the filter cake was dried at 50°C after adding antioxidants. 3.13 g of lutein crystal powder was obtained, of which the UV content of lutein was 90.20%, and the content of all-trans lutein was 92.75% through HPLC detection, and the pure yield of lutein was 85.27%.

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Abstract

The invention provides a method for preparing a food-grade lutein crystal from a marigold extract. The method comprises the following steps of: performing a saponification reaction on the marigold extract in a food-grade lower alcohol solvent by adding a solid alkali; diluting; then cooling and crystallizing; regulating the pH value; filtering; washing by adding an alcohol-water solution; and drying by adding a small amount of antioxidant, thereby obtaining lutein crystal powder. According to the method, the process is simple; the adopted solvent is recycled after being distilled, so that the treatment cost is low. Thus, the method is liable to industrial production. As the method is used, the defects of the existing lutein preparation method that the cost is high, the process is complicated, the time consumption is long and the lutein ester is unstable by performing reactions via harmful organic solvents under an acidic condition and at a high temperature are overcome. For the prepared lutein crystal, the actual yield is greater than 85%; and the purity reaches 80% to 90%. The prepared lutein crystal can be further prepared into an oil suspension or micro capsule powder which is applied to the fields of food additives, food supplements, functional foods and the like.

Description

technical field [0001] The invention relates to the research field of production technology of natural pigments, in particular to a method for preparing high-yield food-grade lutein crystals from marigold extract. Background technique [0002] Lutein widely exists in humans and animals and plants in nature. Humans and animals cannot synthesize it by themselves. The lutein synthesized in plants mainly exists in the form of fatty acid esters. Humans take in lutein esters from fruits and vegetables through diet, hydrolyze into lutein monomers in the small intestine, enter the blood circulation, and accumulate in various organs to play a role. Studies have shown that lutein has many functions such as coloring, anti-oxidation, eye protection, anti-cardiovascular disease and cancer, and is widely used in food, medicine, eye health products, cosmetics and aquaculture. In recent years, the market for lutein products has grown rapidly. BCC's research report pointed out that its mark...

Claims

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Application Information

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IPC IPC(8): C07C403/24
Inventor 吴文忠薜凌云徐维锋李倩
Owner INNOBIO CORP LTD
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