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Method for extracting tryptophan by use of 1,3-bis-(2-carboxyethyl)imidazole tetrafluoroborate ionic liquid supported hydrophilic mixed cellulose liquid membrane

A technology of imidazolium tetrafluoroborate and mixed cellulose membrane, which is applied in chemical instruments and methods, membrane technology, semi-permeable membrane separation, etc., to achieve the effect of simple process, environmental friendliness and high extraction efficiency

Active Publication Date: 2014-12-31
DALIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the method of extracting amphoteric functional group substance amino acid with ionic liquid supported liquid membrane has not been reported.

Method used

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  • Method for extracting tryptophan by use of 1,3-bis-(2-carboxyethyl)imidazole tetrafluoroborate ionic liquid supported hydrophilic mixed cellulose liquid membrane
  • Method for extracting tryptophan by use of 1,3-bis-(2-carboxyethyl)imidazole tetrafluoroborate ionic liquid supported hydrophilic mixed cellulose liquid membrane

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Experimental program
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Effect test

Embodiment 1

[0023] A 0.10mmol / L tryptophan aqueous solution (pH=1 adjusted with concentrated hydrochloric acid) was used as the donor phase, and a 0.10mol / L NaOH solution (pH=13) was used as the acceptor phase. The stirring time is 200min, and the stirring speed is 400rpm. 1,3-bis-(2-carboxyethyl)imidazolium tetrafluoroborate ionic liquid supported hydrophilic mixed cellulose liquid membrane for extraction of tryptophan, using a UV spectrophotometer to detect the donor phase and acceptor at a wavelength of 278nm The concentration change of tryptophan before and after phase extraction calculated the extraction rate as 84.6%.

Embodiment 2

[0025] A 0.40mmol / L tryptophan aqueous solution (pH=1 adjusted with concentrated hydrochloric acid) was used as the donor phase, and a 0.10mol / L NaOH solution (pH=13) was used as the acceptor phase. The stirring time is 200min, and the stirring speed is 400rpm. 1,3-bis-(2-carboxyethyl)imidazolium tetrafluoroborate ionic liquid supported hydrophilic mixed cellulose liquid membrane for extraction of tryptophan, using a UV spectrophotometer to detect the donor phase and acceptor at a wavelength of 278nm The concentration change of tryptophan before and after phase extraction calculated the extraction rate as 23.5%.

Embodiment 3

[0027] A 0.50mmol / L tryptophan aqueous solution (pH=1 adjusted with concentrated hydrochloric acid) was used as the donor phase, and a 0.10mol / L NaOH solution (pH=13) was used as the acceptor phase. The stirring time is 200min, and the stirring speed is 400rpm. 1,3-bis-(2-carboxyethyl)imidazolium tetrafluoroborate ionic liquid supported hydrophilic mixed cellulose liquid membrane for extraction of tryptophan, using a UV spectrophotometer to detect the donor phase and acceptor at a wavelength of 278nm The concentration change of tryptophan before and after phase extraction calculated the extraction rate as 22.2%.

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Abstract

The invention relates to a 1,3-bis-(2-carboxyethyl)imidazole tetrafluoroborate ionic liquid supported hydrophilic mixed cellulose liquid membrane and a method for extracting tryptophan. A carrier supporting a liquid membrane is a hydrophilic mixed cellulose membrane; the hydrophilic mixed cellulose membrane is filled with 1,3-bis-(2-carboxyethyl)imidazole tetrafluoroborate ionic liquid to form a 1,3-bis-(2-carboxyethyl)imidazole tetrafluoroborate ionic liquid supported liquid membrane; the 1,3-bis-(2-carboxyethyl)imidazole tetrafluoroborate ionic liquid supported liquid membrane is mounted in a reaction device, and the donor phase is 0.10-0.50mmol / L tryptophan aqueous solution; the pH is adjusted to 1-5 by concentrated hydrochloric acid, and the acceptor phase is 0.01-0.10mol / L sodium hydroxide aqueous solution (pH=9-13); in the reaction device, stirring lasts for 100-300 minutes at a speed of 200-1,000rpm, and the effective membrane area is about 4-6cm<2>; and the concentration of tryptophan in the donor phase and the concentration of tryptophan in acceptor phase are quantitatively detected by an ultraviolet spectrophotometer, wherein the ultraviolet detection wavelength is 278nm, and the tryptophan extraction efficiency can reach 22.2-84.8%. The method provided by the invention has the advantages of environmental friendliness, mild operation conditions, simple flow and low cost and is an efficient and green tryptophan separation method.

Description

technical field [0001] The invention belongs to the field of chemical separation, and is a new method for extracting tryptophan by using a supported liquid membrane. Specifically, it relates to a method for extracting and separating tryptophan by using polycarboxy functionalized ionic liquid and hydrophilic mixed cellulose membrane. Background technique [0002] Amino acids are an important class of amphoteric functional group compounds, which have important physiological functions and are widely used in the food and pharmaceutical industries. At the same time, amino acids are also intermediates for the synthesis of special chemical substances, such as low-calorie sweeteners, chelating agents, and polypeptides. At present, most amino acids are produced by fermentation, and the cost of separation and purification accounts for about 80% of the total cost. Therefore, improving the selectivity and extraction rate of amino acid separation has extremely important practical signif...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D71/10B01D69/10B01D61/38C07D209/10
Inventor 李长平尹静梅姚志龙王爱玲崔颖娜贾颖萍
Owner DALIAN UNIV