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Bacillus cereus strain B2, liquid preparation, preparation method of liquid preparation and application in controlling ellinia trunk rot of hybridized bamboo

A technology of bacillus cereus and liquid preparations, which is applied in the field of prevention and control of hybrid bamboo white spot feather disease, and can solve the problems of drug resistance and phytotoxicity

Inactive Publication Date: 2013-10-23
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although chemical control is an effective control method, there are many practical problems such as pathogen resistance, environment and food safety, and drug damage. Some chemical fungicides have been strictly restricted in many developed countries and regions.

Method used

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  • Bacillus cereus strain B2, liquid preparation, preparation method of liquid preparation and application in controlling ellinia trunk rot of hybridized bamboo
  • Bacillus cereus strain B2, liquid preparation, preparation method of liquid preparation and application in controlling ellinia trunk rot of hybridized bamboo
  • Bacillus cereus strain B2, liquid preparation, preparation method of liquid preparation and application in controlling ellinia trunk rot of hybridized bamboo

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Embodiment Construction

[0006] The present invention will be described in detail below in conjunction with specific embodiments.

[0007] (1) Screening of Bacillus cereus B2 strain

[0008] 1.1 Pathogens tested

[0009] The pathogenic bacteria tested was the hybrid white-striped feather fungus [Rosellinianecatrix (Hart.) Berl.].

[0010] 1.2 Isolation and purification of spores

[0011] Take the hybrid bamboo rhizosphere soil (below the surface layer 10cm) and put it into a sterile sample bag and bring it back to the laboratory for future use. Weigh 10 per sample g Transfer to a Erlenmeyer flask filled with 90 mL of normal saline and a small amount of glass beads, mix thoroughly to make a soil suspension, and heat in a 100°C water bath for 5 minutes. After cooling, take 1mL under aseptic conditions and add it to a test tube filled with 9mL sterile water, and mix well to obtain 10 -2 The sample diluents were diluted sequentially according to this method to obtain 10 -3 、10 -4 、10 -5 and 10 -6...

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Abstract

The invention discloses bacillus cereus strain B2, a liquid preparation, a preparation method of the liquid preparation and an application in controlling rosellinia necatrix of hybridized bamboo. The strain has been preserved in China General Microbiology Center of Committee for Culture Collection of Microorganisms (CGMCC), Chaoyang District, Beijing, China, since 20th June, 2013, and has a preservation number of CGMCC No.7767 and a classified name of bacillus cereus. The above liquid preparation is diluted by 100-200 times (volume ratio) and is poured to the root of each of non-pathogeny hybridized bamboos along root system breadth direction with 100 mL per plant and once in spring every year, thereby the generation of rosellinia necatrix can be prevented. The above liquid preparation is diluted by 50-100 times (volume ratio) and is poured to the root of each of morbidity hybridized bamboos along root system breadth direction with 200 mL per plant and once in spring and once in summer every year for three successive years, thereby rosellinia necatrix can be effectively cured.

Description

technical field [0001] The invention relates to a Bacillus cereus B2 strain, a liquid preparation and a preparation method thereof, and an application in preventing and treating hybrid bamboo white spot feather disease. Background technique [0002] Supporting green hybrid bamboo is an important economic timber bamboo in the south, and it is also one of the main economic bamboo species introduced and developed in Sichuan and other places. In recent years, the white-striped feather disease of hybrid bamboo caused by Rosellinianecatrix (Hart.) Berl. has caused root rot and the death of the whole bamboo plant, resulting in large economic losses and greatly threatening the return of farmland in the upper and middle reaches of the Yangtze River. Reforestation process and ecological barrier construction. At present, chemical control and silviculture techniques are mainly used, while research on biological control measures has not yet been involved. Although chemical control is a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/085
Inventor 朱天辉李姝江邵宝林张静韩珊谯天敏郑磊张丽娜
Owner SICHUAN AGRI UNIV
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