Bacillus cereus strain B2, liquid preparation, preparation method of liquid preparation and application in controlling ellinia trunk rot of hybridized bamboo
A technology of bacillus cereus and liquid preparations, which is applied in the field of prevention and control of hybrid bamboo white spot feather disease, and can solve the problems of drug resistance and phytotoxicity
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[0006] The present invention will be described in detail below in conjunction with specific embodiments.
[0007] (1) Screening of Bacillus cereus B2 strain
[0008] 1.1 Pathogens tested
[0009] The pathogenic bacteria tested was the hybrid white-striped feather fungus [Rosellinianecatrix (Hart.) Berl.].
[0010] 1.2 Isolation and purification of spores
[0011] Take the hybrid bamboo rhizosphere soil (below the surface layer 10cm) and put it into a sterile sample bag and bring it back to the laboratory for future use. Weigh 10 per sample g Transfer to a Erlenmeyer flask filled with 90 mL of normal saline and a small amount of glass beads, mix thoroughly to make a soil suspension, and heat in a 100°C water bath for 5 minutes. After cooling, take 1mL under aseptic conditions and add it to a test tube filled with 9mL sterile water, and mix well to obtain 10 -2 The sample diluents were diluted sequentially according to this method to obtain 10 -3 、10 -4 、10 -5 and 10 -6...
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