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Application of OsOAT gene to control drought resistance and heavy-metal mercury resistance of rice

A technology of anti-heavy metals and genes, applied in the fields of application, genetic engineering, plant genetic improvement, etc., to achieve the effect of improving drought resistance

Inactive Publication Date: 2013-11-06
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the relationship between proline accumulation and plant stress resistance has been clear, it has not been reported whether the rice ornithine aminotransferase gene OsOAT can improve rice stress resistance.

Method used

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  • Application of OsOAT gene to control drought resistance and heavy-metal mercury resistance of rice
  • Application of OsOAT gene to control drought resistance and heavy-metal mercury resistance of rice
  • Application of OsOAT gene to control drought resistance and heavy-metal mercury resistance of rice

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Embodiment Construction

[0020] The following examples define the present invention and describe the method for cloning a DNA fragment containing the entire coding segment of the OsOAT gene and verifying the function of the OsOAT gene. Based on the following description and these embodiments, those skilled in the art can determine the basic characteristics of the present invention, and without departing from the spirit and scope of the present invention, can make various changes and modifications to the present invention to make it applicable differently. Uses and conditions.

[0021] 1. Detect the expression level of endogenous OsOAT genes in rice

[0022] The applicant selected the japonica rice variety "Zhonghua 11" (a rice variety cultivated by the Institute of Crop Science, Chinese Academy of Agricultural Sciences) as a material for expression profile analysis. The seedlings of rice Zhonghua 11 (ZH11) grown to the 4-leaf stage were subjected to various stress treatments. Drought treatment is to let ...

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Abstract

The invention belongs to the field of rice gene engineering technology, and specifically relates to an application of OsOAT gene to control drought resistance and heavy-metal mercury resistance of rice, wherein the OsOAT gene is obtained through separation, clone and function verification. The OsOAT gene has a nucleotide sequence represented by SEQ ID NO: 1 and a protein sequence represented by SEQ ID NO: 2. By employing a candidate-gene screening method, the OsOAT gene with rice drought resistance is cloned; and through overexpression of the OsOAT gene, transgenic rice is improved in drought resistance and heavy-metal mercury resistance, and the functions and the application of the gene are verified.

Description

Technical field [0001] The invention belongs to the technical field of rice genetic engineering. Specifically, it relates to the application of an OsOAT gene that can improve the drought tolerance and the resistance to heavy metal mercury in rice genetic improvement through isolation, cloning and functional verification. The invention adopts a candidate gene screening method to clone the rice drought resistance gene OsOAT. By overexpressing the OsOAT gene, the ability of transgenic rice to resist drought and heavy metal mercury can be improved, and the function and application of the gene are confirmed. Background technique [0002] Plants are affected by many environmental factors during the growth process. Drought, cold damage and high temperature will cause large-scale reduction in crop production, which is a bottleneck for agricultural development in many areas. Cultivating stress-tolerant crop varieties has always been one of the main goals of agricultural science and techn...

Claims

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Application Information

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IPC IPC(8): C12N15/54C12N9/10C12N15/82A01H5/00
Inventor 熊立仲游均
Owner HUAZHONG AGRI UNIV