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Method for propagating wild Vietnamese sophora root through cuttage

A technology of wild bean and bean, which is applied in the field of plant cultivation, can solve the problems of difficult large-scale practicality, industrialization, low survival rate of wild bean roots, and high cost of transplanting test-tube seedlings, achieving low production cost, Easy to be applied on a large scale and solve the effect of easy pollution from migration

Active Publication Date: 2013-11-20
安顺市宝林科技中药饮片有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The survival rate of wild bean root is low, and the cost of transplanting test-tube seedlings is relatively high, the survival rate of external transplantation is low, and the cost of tissue culture expansion is high, which is difficult for large-scale practical and industrialization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Cutting of wild bean rhizome:

[0026] Cut the stems of wild bean root into 11cm budded stem segments, with 4 buds per segment; put the budded stem segments on the ultra-clean workbench, put them in 0.1% mercury chloride solution for disinfection for 4 minutes, take them out and then Rinse with 70% alcohol, then rinse 4 times with sterile water;

[0027] (2) Put the stem segments into the nutrition bag:

[0028] 2 / 3 of the stem section cut out in step (1) is implanted into the nutrition bag, and 1 / 3 is exposed outside the nutrition bag. There are 3 buds on the stem section of the implanted nutrition bag, and the nutrition bag is equipped with pH The humus soil with a value of 6.5; the humus soil in the nutrition bag is made by putting the black oil sand soil with a pH value of 6.5 into a dry pot and roasting at a high temperature of 75°C;

[0029] (3) Incubation for 20 days:

[0030] Place the nutrient bag with the stem section in step (2) in an environment with ...

Embodiment 2

[0035] (1) Cutting of wild bean rhizome:

[0036] Cut the stem of the wild bean root into 10cm section with buds, each section has 3 buds;

[0037] (2) Put the stem segments into the nutrition bag:

[0038]2 / 3 of the stem section cut out in step (1) is implanted in the nutrition bag, and 1 / 3 is exposed outside the nutrition bag. There are 2 buds on the stem section implanted in the nutrition bag, and the nutrition bag is equipped with pH Humus with a value of 6;

[0039] The humus soil in the nutrition bag in the step (2) is obtained by putting the black oil sand soil with a pH value of 6 into a dry pot and roasting at a high temperature of 70°C;

[0040] (3) Incubation for 19 days:

[0041] Place the nutrient bag with the stem section in step (2) in an environment with a temperature of 15°C and a humidity of 50%, water it once every 2 days, and cultivate it for 19 days to obtain rooted seedlings of the bean root;

[0042] (4) Move into Daejeon:

[0043] The rooted seedli...

Embodiment 3

[0045] (1) Cutting of wild bean rhizome:

[0046] Cut the stem of wild bean root into 12cm section with buds, each section has 4 buds;

[0047] (2) Put the stem segments into the nutrition bag:

[0048] 2 / 3 of the stem section cut out in step (1) is implanted into the nutrition bag, 1 / 3 is exposed outside the nutrition bag, there are 3 buds on the stem section implanted in the nutrition bag, and the nutrition bag is equipped with pH Humus with a value of 7;

[0049] The humus soil in the nutrition bag in the step (2) is prepared by putting the black oil sand soil with a pH value of 7 into a dry pot and roasting at a high temperature of 80°C;

[0050] (3) Incubation for 21 days:

[0051] Place the nutrient bag with the stem section in step (2) in an environment with a temperature of 20°C and a humidity of 70%, water it once every 2 days, and cultivate it for 21 days to obtain rooted seedlings;

[0052] (4) Move into Daejeon:

[0053] The rooted seedlings of the bean root c...

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Abstract

The invention relates to a method for propagating a wild Vietnamese sophora root through cottage. The method comprises the following steps that (1) a wild Vietnamese sophora rootstalk is cut, (2) rootstalk sections are placed into nutrient bags, (3) heat-preservation cultivation is conducted for 19-21 days, and (4) seedlings are transplanted to a field. The method solves the problems that in traditional tissue culture, the requirements for indoor propagating conditions are high, and pollution is prone to occurring during transplanting and provides a good basis for vegetative propagation of Vietnamese sophora root plants. Moreover, large-scale practicability realization and industrialization are easy, and a technical path which realizes high survival rate, low cost and strong practicability is provided for large scale tissue culture propagation, production and development in the future biotechnological field.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a method for cutting and propagating wild bean roots. Background technique [0002] Vietnamese Sophora Root (Vietnamese Sophora Root) is a plant of the genus Sophora in the dicotyledonous class Fabaceae. It is also known as Vietnamese Sophora, Guangdougen, and Hudoulian. It is mainly distributed in Guangxi, Guizhou, and Yunnan. Shandougen is a shrub with a height of 1-2m. It grows in limestone mountains or rock crevices at an altitude of 1000-2000m in subtropical or temperate zones. It grows in red soil or yellow soil, with a pH value of 4.5-5.5. Therefore, the artificial cultivation of wild bean root is not easy to realize. The dried roots and rhizomes of Shandougen are common traditional Chinese medicines, which can clear fire and detoxify, reduce swelling and relieve pain. The extract of the mountain bean root also has anti-tumor, anti-pathogenic microorganisms an...

Claims

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Application Information

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IPC IPC(8): A01G1/00
Inventor 于以祥于轩俊周维书董其洪
Owner 安顺市宝林科技中药饮片有限公司
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