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Extraction method of high-purity coptis total alkaloid

A total alkaloid, high-purity technology, applied in the direction of drug combination, pharmaceutical formula, plant raw materials, etc., can solve the problem of not considering the recovery of alkaloids, not knowing how much alkaloids are taken away by precipitation, and not thinking about recycling alkaloids and other issues, to achieve the effect of low product cost, full utilization of resources, and cost reduction

Active Publication Date: 2015-02-25
重庆伊士腾生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a large amount dissolved in the solution does not necessarily mean a large amount in the end, because there will be various losses in the subsequent processing process, and some losses will be very large
[0016] From the reports of the prior art, it can be seen that the prior art does not consider the recovery of alkaloids in the mother liquor and the recovery of the alkaloids taken away by the precipitation, mainly because there are blind spots in the research: that is, many researchers have not carried out systematic analysis and do not It is clear how much alkaloid is taken away by precipitation and how much alkaloid is taken away by mother liquor, that is, it is not clear at which step the alkaloid is lost, so the problem of alkaloid recovery has not been thought about

Method used

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  • Extraction method of high-purity coptis total alkaloid

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Effect test

Embodiment 1

[0058] Grind or slice 1 kg of Coptis chinensis, add 3 liters of 5% sulfuric acid solution, soak for 1 hour, heat up to 100°C for reflux extraction for 0.1 hour, filter; then use 3 liters of 5% sulfuric acid solution to heat up to 100°C and extract three times; combine The first and second extractions (three extractions and four extractions are used for the processing of the next batch of raw materials), and the supernatant is separated after cooling (or filtered to obtain the supernatant); the supernatant is made of lime (calcium oxide ) neutralize to pH=2, filter, and wash the precipitate 3 times with 0.1 times the precipitation amount of water; use concentrated hydrochloric acid to adjust the pH of the solution to about 1, add 1% sodium chloride and stir well at room temperature (30 ℃) stand still for 48 hours; filter and wash the precipitate three times with an appropriate amount of water to obtain the total alkaloids; combine the mother liquor and the washing solution of th...

Embodiment 2

[0060] Add 10 liters of 1% sulfuric acid solution to 1 kg of coptis root, soak for 12 hours, heat up to 60°C to extract for 1 hour, filter; then use 10 liters of 1% sulfuric acid solution to heat up to 60°C and extract three times; combine the first and the second extraction liquid (three extractions and four extractions are used for the processing of the next batch of raw materials), and the supernatant is separated after cooling (or filtered to obtain the supernatant); the supernatant is neutralized with lime (calcium oxide) To pH = 12, filter, and wash the precipitate once with 3 times the amount of precipitation; the neutralization solution is concentrated 5 times under reduced pressure, filtered, and the filtrate is adjusted to pH = 5 with concentrated hydrochloric acid, and 10% sodium chloride is added After fully stirring, stand still at low temperature (0°C) for 1 hour; filter, and wash the precipitate with appropriate amount of water three times to obtain the total alk...

Embodiment 3

[0062] Add 15 liters of 0.1% sulfuric acid solution to 1 kg of Coptidis ash, soak for 48 hours, heat up to 20°C (if the temperature exceeds 20°C, you don’t need to heat up), extract for 5 hours, and filter; then use 15 liters of 0.1% sulfuric acid for the residue Warm up the solution to 20°C and extract three times; combine the first and second extracts (three extractions and four extractions are used for the processing of the next batch of raw materials), and separate the supernatant after cooling (or filter to obtain the supernatant) The supernatant is neutralized to pH=6 with lime (calcium oxide), filtered, and the precipitate is washed twice with water of 1 times the amount of precipitation; the neutralization solution is concentrated 20 times under reduced pressure, filtered, and the filtrate is adjusted with concentrated hydrochloric acid. pH = about 3, add 20% sodium chloride and stir thoroughly, and then stand still at low temperature (10°C) for 12 hours; filter, and wa...

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Abstract

The invention relates to an extraction method of high-purity coptis total alkaloid, which comprises the following steps: preparing a raw material (coptis or coptis fibril or coptis ash), pulverizing (not required if the raw material is coptis fibril or coptis ash), immersing in 0.1-5% sulfuric acid, heating and extracting four times, filtering, neutralizing with calcium oxide (or calcium hydroxide), filtering, washing the precipitate, preconcentrating, regulating the acid, precipitating with sodium chloride, filtering, recovering alkaloid from the mother liquor, and drying the precipitate to obtain the coptis total alkaloid product. The main active components of the product are coptis effective components, such as berberine, coptisine, palmatine, epiberberine, jateorhizine and the like; the coptis total alkaloid content is more than 50%; and the berberine content is more than 25%. The product provided by the invention has favorable actions of lowering blood sugar and blood fat, losing weight and the like, and is an ideal raw material for treating diabetes and cardiovascular diseases.

Description

technical field [0001] The invention belongs to biological extraction technology, in particular to a high-purity Coptis chinensis total alkaloid extraction technology. Background technique [0002] Coptis chinensis was first recorded in "Shen Nong's Materia Medica" and listed as top grade. Coptis chinensis is bitter in taste, cold in nature, and has the functions of purging fire, detoxifying, clearing away heat, and drying dampness. For dysphoria, confusion, upset and insomnia, hot and humid fullness, vomiting, abdominal pain and diarrhea, conjunctival congestion and swelling, sore tongue, eczema, burns, vomiting blood, epistaxis, etc. The results of modern pharmaceutical research show that the main active ingredient of Coptis chinensis is the total alkaloids of Coptis chinensis, mainly including berberine (berberine), palmatine, coptisine, epiberberine and jatrorrhizine, etc. ("Chinese Pharmacopoeia" 2010 edition) ; Coptis (sugar) has a wide range of pharmacological activ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D455/00A61K36/718A61P3/10A61P3/06A61P3/04A61P9/00
Inventor 叶小利李学刚
Owner 重庆伊士腾生物科技有限公司
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