Sugarcane genome endogenous reference gene isopentenyl diphosphate delta-isomerase gene PCR (polymerase chain reaction) primer sequences and amplification method

A technology of prenyl diphosphate and internal standard gene, which is applied in recombinant DNA technology, DNA/RNA fragments, DNA preparation, etc., can solve the problem that there is no research report on copy number, and achieve easy determination, strong specificity, The effect of wide applicability
CN103409412BInactive Publication Date: 2015-02-04FUJIAN AGRI & FORESTRY UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
FUJIAN AGRI & FORESTRY UNIV
Publication Date
2015-02-04
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses sugarcane genome endogenous reference gene isopentenyl diphosphate delta-isomerase gene PCR (polymerase chain reaction) primer sequences and an amplification method. The primer sequences are composed of ScIDI2-F and ScIDI2-R. The PCR amplification method comprises a reaction system and amplification conditions. The invention provides a genome endogenous reference gene for PCR detection and species specific identification of transgenic sugarcane, and meanwhile, provides insurance for detection precision and result accuracy of the transgenic sugarcane.
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Description

technical field

[0001] The invention belongs to the field of biological technology, and in particular relates to a PCR primer sequence and an amplification method of an internal standard gene prenyl diphosphate delta-isomerase gene of sugarcane genome. Background technique

[0002] Transgenic technology is becoming more and more mature, and the types of genetically modified sugarcane are becoming more and more diverse. At present, herbicide-resistant, insect-resistant, and disease-resistant genetically modified sugarcane has been field tested in 14 countries including the United States, Australia, India, and Brazil, creating conditions for the commercial cultivation of genetically modified sugarcane. In my country, sugarcane gene cloning and transgenic research only started in the mid-1990s. Although it started late, it has made rapid progress. The transgenic sugarcane that has been obtained includes insect-resistant genetically modified sugarcane, drought-resistant genetica...

Claims

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