Alga sort classification identification method based on Raman spectroscopy technique

Abstract

The invention discloses an alga sort classification identification method based on a Raman spectroscopy technique. The alga sort classification identification method comprises the steps as follows: a plurality of samples of the same alga sort are taken, each sample is living alga liquid of the current alga sort, and the Raman spectroscopy original information about the each sample is obtained by adopting a Raman spectrograph; the acquired Raman spectroscopy original information is preprocessed to obtain a corresponding preprocessing spectral graph, and main factors are abstracted from each preprocessing spectral graph by adopting the partial least squares; the alga sort is exchanged, and main factors corresponding to different alga sorts are obtained; a BP nerve network model is established via taking the main factors of all the alga sorts as the input and taking the classification of the alga sort corresponding to the each main factor as the output; a living alga liquid to be identified is taken to acquire the main factors of the living alga liquid to be identified, and input the main factors into the BP nerve network model, and the alga sorts contained in the living alga liquid to be identified are obtained. The alga sort classification identification method achieves quick and accurate classification of alga sorts based on the Raman spectroscopy technique, greatly simplifies operation steps and shortens detection time.

Description

technical field [0001] The invention relates to the technical field of classification and detection of algae species, in particular to a method for classification and identification of algae species based on Raman spectroscopy. Background technique [0002] Microalgae are a class of aquatic (or terrestrial, aerial, symbiotic) lower plants with diverse phylogenies, small individuals, usually single cells or groups, and capable of photosynthesis (a small part is heterotrophic growth). It is a microorganism capable of photosynthesis, using sunlight, water and CO 2 Synthesize the substances you need. At present, the identification, classification and biochemical analysis of algae have become one of the research hotspots in marine biology. [0003] Chlorella (Chlorella) is a kind of single-celled green algae, which belongs to Chlorophyta, Chlorophyceae, and Chlorella genus. It is widely distributed in nature and has the most species in freshwater waters. Chlorella is easy to c...

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Problems solved by technology

[0006] The invention provides a method for classifying and identifying algae species based on Raman spectroscopy technology, which solves the problems of low detection rate, relatively cumbersome operation, time-consuming and labor-consuming problems of existing detection methods

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