Rhesus monkey diabetic nephropathy model preparation method

The technology of diabetic nephropathy and rhesus monkey is applied in the field of preparation of rhesus monkey diabetic nephropathy model, which can solve the problems of poor drug treatment effect and the like, and achieve the effects of strong repeatability and simple administration method.

Active Publication Date: 2013-12-11
WEST CHINA HOSPITAL SICHUAN UNIV +1
View PDF5 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Once DN patients have significant clinical symptoms, there are fewer drugs to choose at this time, and the therapeutic effect of drugs is poor, and the disease often develops progressively until end-stage renal failure, which brings a huge economic burden to the patient's family and society.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rhesus monkey diabetic nephropathy model preparation method
  • Rhesus monkey diabetic nephropathy model preparation method
  • Rhesus monkey diabetic nephropathy model preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 Preparation of rhesus monkey diabetic nephropathy model of the present invention

[0035] 1 Experimental materials and instruments

[0036] 1.1 Basic information of the test product

[0037] IL-1β antibody: abcam

[0038] TNF-a antibody: abcam

[0039] Antibody to TGF-β1: Bioworld

[0040] CTGF antibody: abcam

[0041] Collagen IV antibody: abcam

[0042] Fibronection Antibody:Bioworld

[0043] Antibody to MCP-1: ebioscience

[0044] CD68(KP1) antibody: abcam

[0045] eNOS antibody: abcam

[0046] Animal Tissue Total RNA Extraction Kit: Tiangen

[0047] iScript TM cDNA synthesis kit: BIO-RAD

[0048] iQ TM Green Supermix: BIO-RAD

[0049] 1.2 The main instruments and equipment required for the test

[0050] Blood glucose detector: Luokang full vitality blood glucose detector, Luokang full vitality blood glucose test strip

[0051] Automatic blood biochemical analyzer (Roche COBAS Integra400Plus, Switzerland, number MEB-02-01)

[0052] BIO-...

Embodiment 2

[0136] Embodiment 2 screens the medicine for the treatment of diabetic nephropathy with the model of the present invention

[0137] a, the rhesus monkey diabetic nephropathy model established according to the method of Example 1;

[0138] b. administering the drug candidate to an animal model;

[0139] c. Observe the effect of candidate drugs on various indicators of metabolic syndrome, and evaluate potential drugs for treating diabetic nephropathy.

[0140] In summary, the modeling method of the present invention induces clinical symptoms of diabetic nephropathy in rhesus monkeys through long-term blood sugar control. In addition, the combination of long-term blood sugar control and high fat intake can also induce clinical symptoms of diabetic nephropathy in rhesus monkeys. symptoms, and the symptoms are more significant, indicating that the two methods of the present invention can effectively establish a rhesus monkey diabetic nephropathy model.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention discloses a rhesus monkey diabetic nephropathy model preparation method, which comprises: (1) applying streptozocin with a dose of 80-100 mg/kg on a rhesus monkey; and (2) after a blood glucose concentration is increased to 11.1 mmol/l, applying insulin before meal, such that a rhesus monkey blood glucose concentration is 10-20 mmol/L. According to the present invention, based on the diabetes mellitus rhesus monkey, the blood glucose control method is adopted to successfully induce the rhesus monkey diabetic nephropathy model.

Description

technical field [0001] The invention relates to a preparation method of a rhesus monkey diabetic nephropathy model. Background technique [0002] The incidence of Diabetes Mellitus (DM) is increasing rapidly. According to reports, there are about 92 million DM and 148 million pre-DM patients in China, becoming the second country with the largest incidence of diabetes after the United States. With the gradual development of the disease, about 30-40% of these patients with type 1 diabetes and about 20% of type 2 diabetes patients are accompanied by severe renal damage-diabetic nephropathy (Diabetic Nephropathy, DN). [0003] DN is one of the most serious chronic complications of diabetic patients and the leading cause of death in diabetic patients. Once DN patients have significant clinical symptoms, there are fewer drugs to choose, and the therapeutic effect of drugs is poor. The disease often develops progressively until end-stage renal failure, which brings a huge economi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/28A61K31/7008A61K49/00A01K67/02A23K1/16A23K1/18
CPCA61K49/0008A01K67/02A01K67/027A01K2207/25A01K2227/106A01K2267/0362A23K50/00A61K38/28
Inventor 陆燕蓉程惊秋陈又南刘敬平刘芳杨立川王莉李宏霞张杰王丹何斯荣王成世史梅梅袁玉佳赵久茗
Owner WEST CHINA HOSPITAL SICHUAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap