Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for carrying out cell density and arrangement controllable culture based on insect wing base

A technology of insects and cells, which is applied in the field of controlled cell density and arrangement based on insect wing substrates. It can solve the problems of simultaneous controllable cell density and arrangement, complex synthesis process, and damage to cell viability, so as to avoid complex process and safety. Problems, Ease of Access, Cell Viability Preservation Effects

Inactive Publication Date: 2013-12-11
SOUTHEAST UNIV
View PDF2 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the synthesis process of many substrate materials is complicated at present, and even involves some toxic substances, and both physical and chemical methods and printing techniques will cause damage to cell viability
In addition, in the current research on cell culture, there is almost no method that can achieve a cell culture method with simultaneous controllable cell density and arrangement.
Therefore, it cannot meet the needs of fields such as medical implants, tissue engineering, especially bone joint tissue engineering.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for carrying out cell density and arrangement controllable culture based on insect wing base

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Cell Density and Arrangement Controlled Culture Based on Morpho Butterfly Wing Substrate

[0028] 1. Acquisition and fixation of Morpho butterfly scales:

[0029] Cut out rectangular morpho butterfly scale wing slices, and fix the wing slices with the back side up on a glass piece of the same size with polystyrene solution.

[0030] 2. Prepare cell suspension:

[0031] Select the fibroblasts of NIH / 3T3 mice, and dilute the fibroblasts with medium to a concentration of 2×10 5 / mL of cell suspension, put the resulting cell suspension into a glass container of a certain size.

[0032] 3. Cell sedimentation:

[0033] Insert the Morpho butterfly lepidopteran obtained in step 1 into the cell suspension prepared in step 2 at an inclination angle of 15° from the horizontal direction, and let stand at 37° C. for several hours.

[0034] 4. Obtain cell arrangement with directional arrangement and density gradient distribution:

[0035] The morpho butterfly scale wings in step...

Embodiment 2

[0037] Culture of two kinds of cells with controllable cell density and arrangement on the same morpho butterfly scale wing

[0038] 1. Acquisition and fixation of Morpho butterfly scales:

[0039] Cut out rectangular morpho butterfly scale wing slices, and fix the wing slices with the back side up on a glass piece of the same size with polystyrene solution.

[0040] 2. Prepare cell suspension:

[0041] Fibroblasts from NIH / 3T3 mice and bone marrow stem cells from MFC mice were selected and diluted with culture medium to a concentration of 2×10 5 / mL of cell suspension, put the resulting cell suspension into glass containers of a certain size.

[0042] 3. Cell sedimentation:

[0043] Insert the Morpho butterfly lepidopteran obtained in step 1 into the mouse fibroblast suspension prepared in step 2 at an inclination angle of 15° from the horizontal direction, let it stand at 37°C for several hours, take it out, and rinse with phosphoric acid flushing solution After several ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for carrying out cell density and arrangement controllable culture based on an insect wing base. The method is implemented by taking an insect scaly wing as a base, the insect scaly wing is obliquely placed in a cell suspension, cells vertically fall on the surface of the insect scaly wing under the action of gravity, and the number of the cells is gradually increased from top to bottom; and the cells on the surface of the insect scaly wing are directionally arranged according to a micro-nano structure on the surface of the insect scaly wing. According to the invention, the insect scaly wing which is taken as the base is easy to obtain and is safe and non-toxic; cell culture with simultaneously controllable cell density and arrangement can be realized, and a series of process complexity and security problems arising from artificial synthetic materials are avoided; and density gradient distribution is formed by the action of gravity, so that the damage of a mechanical acting force on cells is avoided, the cell vitality is kept, and formed cell arrangement with a directional arrangement rule and density gradient distribution has a potential application prospect and practical significance for tissue regeneration including connection tissue regeneration and restoration.

Description

technical field [0001] The invention belongs to the technical field of biological materials and tissue engineering, and in particular relates to a controllable culture method based on the cell density and arrangement of insect wing substrates. Background technique [0002] With the development of organ and tissue repair, transplantation, and biosensor research in tissue engineering, the directional arrangement and growth of cells and the gradient distribution of cell density are becoming more and more important. At present, with the maturity of micro-nano technology, the substrate materials for cell culture can achieve controllable cell adhesion, directional arrangement and other behaviors in vitro. At the same time, the gradient distribution of cell density can also be achieved in vitro by using mechanical force, physical and chemical methods, and inkjet printer technology. [0003] However, the synthesis process of many base materials is complicated, and even involves som...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/071C12N5/079
Inventor 赵远锦陆洁刘慈慧郑付印顾忠泽
Owner SOUTHEAST UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com