Porcine circovirus type II strain

A porcine circovirus and strain technology, applied in the direction of virus/bacteriophage, microorganism, biochemical equipment and methods, etc., can solve the problem of variable reaction results of animal models of virulent infection, low PCV2 virus proliferation titer, and weak virus proliferation ability And other issues
CN103436498AInactive Publication Date: 2013-12-11HENAN AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HENAN AGRICULTURAL UNIVERSITY
Publication Date
2013-12-11
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention discloses a porcine circovirus type II strain, wherein the latin name is Circoviridae, and the preservation number is CCTCC NO:V201312. According to the invention, in vitro PCV2 culture proliferation conditions are optimized, and a PK15 cell clone strain with high PCV2 sensitivity and named L8 is screened so as to establish a foundation for further PCV2 researches.
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Description

technical field

[0001] The invention relates to a type Ⅱ strain of porcine circovirus. Background technique

[0002] In 1974, a German researcher discovered a new, non-pathogenic microribonucleotide virus-like pollutant from the pig kidney cell line PK15 (ATCC-CCL33). It was subsequently reported that the pollutant was an icosahedral virus with no envelope and a single-stranded circular DNA genome, which was the smallest known virus capable of self-replication in mammalian cells, and was subsequently named pig round Cyclovirus (PCV). It mainly infects monocyte / macrophage lineage cells, among which porcine alveolar macrophages are its main target cells. Subsequent serological surveys of PCV antibodies in pigs in Germany, Canada, Great Britain and Northern Ireland showed that PCV is ubiquitous in pig herds. Experimental infection of 9-month-old pigs with PCV resulted in no clinical disease and the virus was therefore considered non-pathogenic.

[0003] In the late 1990s, a...

Claims

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