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Molecular marker used for assistant selection of brown cotton fiber color gene Lc1 and use of molecular marker

A molecular marker, assisted selection technology, applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of narrow genetic basis, low polymorphism, complicated RFLP operation, etc., and achieve clear selection goals , the effect of low efficiency and cost reduction

Inactive Publication Date: 2013-12-11
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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AI Technical Summary

Problems solved by technology

However, due to the relatively narrow genetic basis of Xinjiang brown cotton, in the commonly used molecular marker system, the RFLP operation is complex and the polymorphism is low, which limits the screening and application of cotton RFLP markers to a certain extent.

Method used

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  • Molecular marker used for assistant selection of brown cotton fiber color gene Lc1 and use of molecular marker
  • Molecular marker used for assistant selection of brown cotton fiber color gene Lc1 and use of molecular marker
  • Molecular marker used for assistant selection of brown cotton fiber color gene Lc1 and use of molecular marker

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Experimental program
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Effect test

Embodiment

[0027] Population construction and trait analysis

[0028] The 5 parental materials of Xinluzao 13, Xinluzao 31, Zhongmiansuo 41, Zhongmiansuo 45 and Xin Caimian 6 were tested in Korla Breeding Test Base of China Colored Cotton (Group) Co., Ltd. in 2007 Planting, reciprocal crossing, get F 1 Generation seeds; In 2008, the breeding experiment base carried out generation, F 1 Generation selfing, get F 2 generation seeds; in 2009, F 2 For generation of seeds, at the seven-leaf stage, collect leaves from individual plants listed on the list, and investigate the color of individual plants after bolling;

[0029] Result analysis: 4 white cottons are fully crossed with 1 brown cotton, no matter orthogonal or reciprocal, F 1 The generation is brown, indicating that brown is dominant over white, and a total of 8 Fs are obtained 2 Generation groups, white and brown separation appeared in the group;

[0030] Fiber Color Genetic Analysis

[0031] in 8 F 2 In generation groups, bro...

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Abstract

The invention relates to a molecular maker used for assistant selection of a brown cotton fiber gene Lc1 and a use of the molecular marker, belonging to the innovative technical field of plant molecular genetics and colored cotton germplasm. The invention relates to a molecular maker developing and positioning method of a brown cotton fiber gene Lc1. The molecular marker linked with the Lc1 gene is firstly obtained, wherein the genetic distance between NAU1043 and Lc1 is 3.85cM, and the genetic distance between the marker NAU2862 and the Lc1 is 7.75cM, wherein both of the genetic distances can be used for assistant breeding of the molecular marker of the gene. The auxiliary breeding selection target is clear and definite by utilizing the NAU1043 and NAU2862 molecular markers disclosed by the invention, so that the breeding time can be saved without being affected by the environment, the efficiency can be improved, and the breeding cost can be lowered. Moreover, the technical base is laid for further map-based cloning of the Lc1 gene.

Description

technical field [0001] The invention belongs to the technical field of plant molecular genetics and colored cotton germplasm innovation, and relates to brown cotton fiber color gene Lc 1 Molecular marker development and its localization method, specifically related to a brown cotton fiber pigment gene Lc 1 Molecular markers for assisted selection and their applications. Background technique [0002] Cotton is one of the most important economic crops in the world and the main raw material for the textile industry. At present, cotton planting is distributed to more than 80 countries in the world, solving the warmth problem of nearly 800 million people. Natural colored cotton is a natural pigment formed during the development of cotton fibers, currently mainly brown and green. Due to its unique natural color, natural colored cotton fiber does not need to be dyed during processing, thus reducing environmental pollution; the underwear processed by it is soft to the touch, comf...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 李晓波王利祥刘海峰肖向文李雪源罗城王俊铎宋武艾先涛
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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