Preparation method and preparation apparatus for typography type tissue chip

Abstract

A method for preparing a loose-type tissue chip and a preparation instrument. The preparation instrument is composed of a cutting module, a micro-wire cutting machine, a wax model of a receptor, and a feeding workbench; Screws and guillotines, positioning holes and missing corners are processed on the bottom plate; the receptor wax model is prefabricated with multiple holes on the entire wax block, and the feeding table includes a horizontal stepping motor and a vertical stepping motor , an encoder, a guide rail and a ball screw; the preparation method includes: 1) preparation of a wax model of a receptor; 2) preparation of a wax core; 3) putting the wax core into the wax model of the receptor; 4) slicing. The invention has the advantages of automatic mechanical operation, accurate positioning, time-saving and labor-saving, small error, high efficiency, easy identification of pole direction, and little damage to sample tissue.

Description

technical field [0001] The invention relates to an important experimental method and device in the fields of biology and medicine, in particular to a preparation method and preparation apparatus of a movable type tissue chip. Background technique [0002] Tissue arrays, also known as tissue microarrays (TMAS), are multiple different tissue samples placed on a single receptor block {receiver block} or paraffin block. Slice the receptor block {receiving block} with a microtome according to the usual method to obtain a specimen. [0003] Tissue microarray technology is a major innovation in experimental technology. Clinical pathologists and biologists use slices of arrayed biological tissues and pathological specimens for analysis to obtain key information on the status and changes of high-throughput biological tissues and pathological specimens. [0004] Through the use of tissue chips, scientists have studied the information of tissue or (and) cell structure, cell function a...

AI Technical Summary

Problems solved by technology

Sometimes when the number of sample tissues is large, this repetitive operation is very time-consuming and laborious

[0007] Most of the existing tissue microarray production techniques use sampling needles. This method has the following disadvantages: most of the sampling needles are used to obtain tissue cores in a blind piercing manner, and the positioning of sampling points is not accurate, and the representativeness of samples is not high; Sampling is performed in a circular manner. Although the impact on the donor tissue block or wax block is small, it is difficult to ensure the integrity of the tissue core taken, and the tissue compression, deformation, and fracture in the prepared wax core are serious; The sampling depth is not easy to control. Although the existing mechanical sampling device can accurately control the puncture depth, when sampling thinner samples, the front end of the wax core may be a blank wax core. Therefore, the tissue cores in the prepared tissue chip are not on the same plane. There are empty spots in the final chip, which reduces the efficiency of chip preparation and is not convenient for the display of tissue samples such as thinner, epithelial and cystic walls.

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