Preparation method for rooting of test-tube plantlet of ample flow pear

Abstract

The invention discloses a preparation method for rooting of a test-tube plantlet of an ample flow pear. The preparation method comprises the following steps of A, carrying out sterile test-tube plantlet cultivation on annual branches and stems of the ample flow pear, namely cutting the sterile test-tube plantlet into branches and stems with axillary buds for disinfection treatment, and cutting a root into an inclined surface to be inserted into an axillary bud medium for cyclic cultivation through day and night illumination; B, carrying out subculture proliferation on the sterile test-tube plantlet, namely cutting axillary bud seedlings which are sterile and robustly grow from the branches and the stems to be inoculated into a subculture medium for cyclic cultivation through the day and night illumination; C, transferring the test-tube plantlet of the ample flow pear into a rooting medium to induce the rooting, namely inoculating the test-tube plantlet of the ample flow pear to a first rooting medium for dark cultivation and then transferring to a second rooting medium for light cultivation to obtain a rooted plant, and carrying out cyclic cultivation through the day and night illumination; D, carrying out transplanting and domestication on the rooted seedlings in an illumination incubator, transferring the survived seedlings into a pot for cultivation. The preparation method is easy to carry out and easy to operate and solves the problem that the test-tube plantlet of the ample flow pear is difficult to root in the process of tissue cultivation; the rooting rate is high, a root system is developed, and the survival rate of transplanting is high.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, and more particularly relates to a preparation method for rooting test-tube seedlings of Fengshui pear, which is suitable for rapidly propagating a large amount of self-rooted test-tube seedlings of Fengshui pear by tissue culture, and meets the needs of the seedling market. Background technique [0002] Fengshui pear (Pyrus pyrifolia'Hosui') is a high-quality large-fruited brown pear variety named in 1972 by the Fruit Tree Experimental Field of the Ministry of Agriculture and Forestry of Japan. one. The fruit weighs 200g-300g, up to 500g. The fruit is round or nearly round, the peel is yellowish brown and bright, and the large fruit has comprehensive grooves. The flesh is snow-white, tender, crisp, juicy, sweet and sour, very good flavor, excellent quality, and more resistant to storage. The fruit development period is 135d-140d, and the short fruiting branches are the main ones. ...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a preparation method for rooting of Fengshui pear test-tube seedlings, which is easy to implement and easy to operate, solves the problem of difficult rooting in the process of tissue culture of Fengshui pear test-tube seedlings, has high rooting rate, well-developed root system and can be transplanted high survival rate

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