Tumor vaccine and preparation method thereof

A tumor vaccine and tumor cell technology, applied in vaccines, anti-tumor drugs, pharmaceutical formulations, etc., can solve the problems of ineffectiveness of tumor antigens, poor targeting and use safety, etc., achieve a broad spectrum of tumor antigens, and enhance targeted lethality , the effect of improving efficiency

Active Publication Date: 2013-12-18
HUBEI SOUNDNY BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention provides an application of cell vesicles in the preparation of tumor vaccines. Cell vesicles derived from apoptotic tumor cells are used as antigens of tumor vaccines, thereby helping to solve the problem that tumor vaccines cannot be effective against all tumor antigens and have poor targeting and issues of security

Method used

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  • Tumor vaccine and preparation method thereof
  • Tumor vaccine and preparation method thereof
  • Tumor vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Tumor cells produce cell vesicles after undergoing apoptosis treatment.

[0050] 1. Experimental materials and reagents

[0051] H22 mouse liver cancer cells, fluorescent dye: carboxyfluorescein diacetate succinimide ester (commercially available, with green fluorescence), and the ultraviolet device is owned by a conventional cell ultra-clean bench.

[0052] 2. Experimental steps

[0053] 1) Culture H22 mouse liver cancer cells in DMEM cell culture medium to make the cell volume reach 2×10 7 ;

[0054] 2) After the H22 mouse liver cancer cells were stained with carboxyfluorescein diacetate succinimide lipid fluorescent dye, fresh culture medium was added, and then the above H22 mouse liver cancer cells were divided into two groups (H22-1 group, H22-1 group, H22-2 group), wherein one group of H22-1 was irradiated with ultraviolet light for 60 minutes, and the other group of H22-2 was not treated and cultured normally in the culture medium;

[0055] 3) 48 h...

Embodiment 2

[0060] Example 2: Cell vesicles produced by induced tumor cells can be taken up by dendritic cells.

[0061] 1. Experimental materials and reagents

[0062] The H22 mouse liver cancer cells and the ultraviolet device used were the same as in Example 1, carboxyfluorescein diacetate succinimidyl ester (CFSE) (green fluorescent dye), PKH26 (red fluorescent dye).

[0063] 2. Experimental steps

[0064] 1) Cultivate H22 mouse liver cancer cells according to the method described in Example 1, so that the cell volume reaches 1×10 7 ; Use carboxyfluorescein diacetate succinimide lipid to stain the above H22 mouse liver cancer cells, then add fresh culture medium, and use ultraviolet radiation for 60 minutes. After 48 hours of ultraviolet radiation, H22 mouse liver cancer cells appear Obviously smaller and dim state, confirm that these tumor cells have been induced apoptosis by ultraviolet rays, and collect the cell vesicles induced by ultraviolet rays with green fluorescence accordi...

Embodiment 3

[0069] Example 3: Dendritic cells up-regulate the expression of co-stimulatory signal molecules after taking up cell vesicles.

[0070] 1. Experimental materials and reagents

[0071] The H22 mouse hepatoma cells used were the same as in Example 1, the dendritic cells were the same as in Example 2, and the fluorescently labeled antibodies against mouse CD80, CD86, and MHC II.

[0072] 2. Experimental steps

[0073] 1) The culture method of dendritic cells is the same as that in Example 2; the cell vesicles of H22 mouse liver cancer cells are prepared by the method in Example 1, and the method for making dendritic cells ingest H22 mouse liver cancer cells is the same as in Example 2.

[0074] 2) The cell vesicles and dendritic cells of H22 mouse liver cancer cells were incubated for 48 hours, and the dendritic cells that had taken up the cell vesicles were used as the experimental group; at the same time, the individual dendritic cells were incubated for 48 hours, as a contro...

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Abstract

The invention provides a tumor vaccine and a preparation method thereof. The tumor vaccine comprises cell vesicles from apoptosis tumor cells and an adjuvant. The invention also provides a preparation method of the tumor vaccine. The method comprises the steps of: irradiating tumor cells by ultraviolet ray to realize tumor cell apoptosis, collecting cell vesicles released by the tumor cells; and mixing the cell vesicles with the adjuvant to form the tumor vaccine. The tumor vaccine provided by the invention comprises wide and comprehensive tumor antigen spectrums, overcomes the defect of incapability to killing wide tumor cells in the prior art, and has good usage security and immunization targeting.

Description

technical field [0001] The invention relates to a vaccine, in particular to a tumor vaccine. Background technique [0002] In recent years, as a disease that seriously threatens people's lives, tumor treatment has become the subject of research by many researchers. In addition to commonly known chemotherapy, surgical resection and other treatments, tumor vaccines are a new type of treatment. means has drawn increasing attention. [0003] Tumor vaccines mainly activate the patient's own immune system, use tumor cells or tumor antigens to induce the body's specific cellular and humoral immune responses, enhance the body's anti-cancer ability, and prevent the growth, spread, and recurrence of tumors to achieve elimination or control tumor purpose. Tumor vaccines can be divided into tumor cell vaccines, gene vaccines and peptide vaccines according to their sources. Tumor cell vaccine is to extract tumor cells from the tumor tissue of the body, after inactivation treatment, th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00A61P35/00
CPCA61K9/0019A61K9/5176A61K39/0011A61K2039/5152A61P35/00A61K2039/80A61K9/009A61K9/14
Inventor 黄波
Owner HUBEI SOUNDNY BIOLOGICAL TECH
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