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Method for extracting ursolic acid from gardenia by using microwaves

A technology of ursolic acid and gardenia, applied in the field of extraction of ursolic acid in gardenia, can solve problems such as few research reports on extraction and refining, and achieve the effects of reducing cost, improving purity and yield, and saving energy.

Inactive Publication Date: 2013-12-18
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the researches on Gardenia jasminoides focus on geniposides, but there are few reports on the extraction and purification of ursolic acid in Gardenia jasminoides

Method used

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  • Method for extracting ursolic acid from gardenia by using microwaves

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Experimental program
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Effect test

Embodiment 1

[0017] Weigh and dry to obtain 1 kg of gardenia residue, add 8L of 80% ethanol, and extract by microwave for 10 minutes. The power of microwave is 200 watts. Extract the crude extract with 10 times the amount of petroleum ether (30~60°C) for 3 times, combine the extracts, filter and concentrate to obtain the extract; add appropriate amount of water to suspend the extract, and pass through the pretreatment at 2BV / h The treated AB-8 macroporous adsorption resin was first eluted with 4 times the amount of distilled water, discarded the eluent, and then eluted with 80% ethanol, collected the eluent, concentrated and dried under reduced pressure, added methanol to crystallize, Obtain 4.21g ursolic acid product, the purity of ursolic acid measured by HPLC method is 89.5%.

Embodiment 2

[0019] Weigh and dry to obtain 1 kg of gardenia residue, add 10 L of 90% ethanol, and extract by microwave for 8 minutes, the power of microwave is 150 watts, after extraction, the solution is filtered, the filtrate is concentrated to dilute extract, and then vacuum-dried to obtain the crude extract of gardenia glycoside, Extract the crude extract twice with 15 times the amount of petroleum ether (30~60°C), combine the extracts, filter, and concentrate to obtain the extract; add an appropriate amount of water to suspend the extract, and pass through the pretreatment at 3BV / h The treated NKA macroporous adsorption resin was first eluted with 2 times the amount of distilled water, discarded the eluent, and then eluted with 70% ethanol, collected the eluent, concentrated and dried under reduced pressure, added methanol to crystallize, and obtained 4.12 g ursolic acid product, the purity of ursolic acid measured by HPLC is 89.1%.

Embodiment 3

[0021] Weigh and dry to obtain 1 kg of gardenia residue, add 12L of 90% ethanol, and extract by microwave for 5 minutes, the power of microwave is 250 watts, after extraction, the solution is filtered, the filtrate is concentrated to dilute extract, and then vacuum-dried to obtain the crude extract of gardenia glycoside, Extract the crude extract with 20 times the amount of petroleum ether (30~60°C) for 4 times, combine the extracts, filter and concentrate to obtain the extract; add an appropriate amount of water to suspend the extract, and pass through the pretreatment at 4BV / h The treated X-5 macroporous adsorption resin was first eluted with 2 times the amount of distilled water, discarded the eluent, and then eluted with 90% ethanol, collected the eluent, concentrated and dried under reduced pressure, added methanol to crystallize, Obtain 4.16g ursolic acid product, and the purity of ursolic acid recorded by HPLC method is 89.3%.

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Abstract

The invention discloses a process for extracting ursolic acid from gardenia by using microwaves. According to the method, the gardenia residue obtained after the geniposide is extracted is used as the raw material, alcohol with certain concentration is used as an extracting agent, and proper solid-to-liquid ratio is regulated, and ursolic acid is extracted with microwaves. The filtering solution is filtered and extracted, and impurities are removed by using macroporous adsorption resin to realize purification; the purified eluant is condensed at reduced pressure and dried, and methanol is crystallized to obtain an ursolic acid product. The purity of the ursolic acid, measured by means of HPLC (High Performance Liquid Chromatography), is higher than 89%. By adopting the method disclosed by the invention, the ursolic acid in the gardenia residue is extracted by means of microwave technology, and the method has the advantages of high efficiency, high speed, time saving, low solvent consumption, low energy consumption and the like, compared with a traditional method.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicines, in particular to a method for extracting ursolic acid from Gardenia jasminoides. Background technique [0002] Gardenia is the dry and mature fruit of Gardenia jasminoides of Rubiaceae. It has been used in clinical treatment in my country for more than 1,600 years. It belongs to the first batch of medicinal and edible resources promulgated by the Ministry of Health. Blood detoxification function. Gardenia fruit contains a variety of effective active ingredients such as geniposide and ursolic acid. Ursolic acid, also known as ursolic acid, belongs to pentacyclic triterpenoids and has a variety of biological activities, including anti-diabetes, hypolipidemic, anti-tumor, anti-oxidation, anti-carcinogenic and so on. Its structure is as follows: [0003] [0004] At present, most of the studies on Gardenia jasminoides focus on geniposides, but there are few reports on the e...

Claims

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Application Information

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IPC IPC(8): C07J63/00
Inventor 苏刘花
Owner NANJING ZELANG AGRI DEV