Preparation method and use method of self-circulation microbial flocculant

A microbial flocculant, self-circulating technology, applied in the direction of dehydration/drying/concentrated sludge treatment, fermentation, etc., can solve the problems of high transportation cost, low concentration, and increased product cost due to processing costs, so as to achieve convenient use and increase costs , low-cost cultivation effect

Inactive Publication Date: 2013-12-18
ZHEJIANG SHUREN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if the flocculant produced by the above method is to be sold commercially and applied industrially, two problems must be solved: (1) the fermentation broth is directly sold as a fl

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0021] Example 1 The flocculating microbial strain A was isolated and screened from the soil of the brewery plant, and 0.5% strain A seed suspension was inoculated into the saccharification workshop wastewater with 0.05% residual sugar after autoclaving, and aeration cultured at 27°C for 48 Hours, the fermentation broth is the flocculant, and the flocculation rate of the flocculant to the kaolin suspension is about 95%. Take 5ml of the flocculant and add it to the 100ml of concentrated sludge in the second settling tank of the brewery sewage treatment workshop. After mixing evenly, flocculation and coagulation will appear. After natural standing and settling for 20 minutes, a clear solid-liquid boundary will appear, indicating that the flocculation dehydration effect is good .

Example Embodiment

[0022] Example 2 The flocculating microbial strain B was separated and screened from the activated sludge of the brewery, inoculated with 2.0% strain B seed suspension into the saccharification workshop wastewater with a residual sugar of 0.05% and pH 6.8, and aerated culture at 35°C 36 Hours, the fermentation broth is the flocculant, and the flocculation rate of the flocculant to the kaolin suspension is about 98%. Take the flocculant 3ml, 1% CaCI 2 2ml was added to 100ml of concentrated sludge in the second settling tank of the brewery sewage treatment workshop. After being evenly mixed, flocculation and coagulation occurred. After natural standing and settling for 20 minutes, a clear solid-liquid boundary appeared, indicating that the flocculation and dehydration effect was good.

Example Embodiment

[0023] Example 3 The flocculating microbial strain C was isolated and screened from the brewery wastewater, inoculated with 1.0% strain C seed suspension into the autoclaved COD: 3000 mg / L, pH 7.0 beer wastewater, and aerated at 35°C After 60 hours of cultivation, the fermentation broth is the flocculant, and the flocculation rate of the kaolin suspension by the flocculant is about 97%. Take the flocculant 3ml, 1% CaCI 2 1ml was added to 100ml of concentrated sludge in the secondary sedimentation tank of the brewery sewage treatment workshop, and flocculation appeared after the mixture was evenly mixed. After standing for 20 minutes, a clear solid-liquid boundary appeared, indicating that the flocculation and dehydration effect was good.

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PUM

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Abstract

The invention relates to a preparation method and a use method of a self-circulation microbial flocculant. The preparation method comprises the following steps of separating and screening a microbial strain having flocculation activity from activated sludge, waste water or soil produced by an organic waste water treatment factory, inoculating a seed medium with the microbial strain to obtain a seed suspension liquid, adjusting a pH value of organic waste water to 5.5-7.5, wherein the organic waste water is used as a fermentation medium directly or after disinfection, inoculating the fermentation medium with 0.5-5% of the seed suspension liquid, and carrying out aeration culture at a temperature of 25-37 DEG C for 24-72h to obtain a bacterium suspension or a supernatant as the self-circulation microbial flocculant. The self-circulation microbial flocculant can be directly used for dehydration of residual sludge and can also be used for dehydration of residual sludge after simple condensation or compounding with other flocculating agents. The preparation method utilizes nutrients in organic waste water to culture flocculation microbes so that the self-circulation microbial flocculant is obtained, and the self-circulation microbial flocculant is reused in residual sludge dehydration in organic waste water treatment so that self-circulation utilization is realized. The preparation method has simple processes, a low cost and good effects, realizes waste treatment by waste, and is conducive to residual sludge recycling application.

Description

technical field [0001] The invention belongs to the field of preparation and application of microbial flocculants, in particular to a preparation and application method of a self-circulating microbial flocculant. technical background [0002] Flocculant is a substance that can coagulate and settle solid suspended particles that are not easy to settle in liquid, and is the main additive for sludge dewatering. There are two main types of traditional flocculants, one is inorganic type, such as iron salt, aluminum salt, etc.; the other is organic type, such as polyacrylamide. The flocculation effects of these two types of flocculants are relatively good, especially polyacrylamide flocculants, which are widely used because of their high flocculation and low production costs. However, in recent years, it has been found that the large amount of inorganic flocculant is likely to cause secondary pollution, and what is more serious is that frequent drinking of water with aluminum sal...

Claims

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Application Information

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IPC IPC(8): C12P1/00C02F11/14
Inventor 蒋新龙蒋益花陈霞蔡成岗张建芬陈虹柯薇
Owner ZHEJIANG SHUREN UNIV
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