Novel immunomic mass spectrometry kit for detecting endogenous and exogenous insulins

Abstract

The invention relates to an immunomic mass spectrometry kit for an endogenous insulin and an exogenous insulin and a preparation method thereof, belonging to the technical field of protein detection. The kit comprises a tube of a standard substance containing C14 isotope labeled endogenous and exogenous insulins, a tube of magnetic beads containing anti-insulin antibody, a tube of a buffer solution, a tube of an eluate, and a tube of an energy absorption molecule saturated solution; and the tubes are arranged in a 4 DEG C refrigeration box. The immunomic mass spectrometry kit can be used for in-vitro sample detection and prognosis judgment. The method is precise, convenient and quick.

Description

technical field [0001] The invention relates to a new method for non-invasive detection and evaluation of diabetic patient kits, which is characterized by the use of mass spectrometry [0002] The method accurately identifies and detects the insulin that has been captured by the specific antibody in the sample. Background technique [0003] Both normal function and pathological characteristics of cells depend to some extent on the function of proteins expressed by cells. Therefore, identifying differences in proteins expressed in humans can be used for screening of disease samples in vitro and ultimately for drug development and disease treatment. However, differential analysis of protein expression and function requires the ability to resolve complex mixtures of intracellular molecules. However, many substances in cells often exist in trace amounts, and the current methods for analyzing proteins have limitations in the above aspects. It is difficult to identify and analyz...

AI Technical Summary

Problems solved by technology

The disadvantage of this methodology is that it is impossible to detect a single amino acid variation of insulin (such as the N or C terminal of insulin missing one or several amino acids, insulin is modified by methyl, acyl, etc., isomers of insulin); The traditional test for insulin is to measure the so-called total insulin

[0008] Because traditional insulin detection is often aimed at the overall level of insulin in patients, it is impossible to directly identify the levels of endogenous insulin and exogenous insulin, which increases the difficulty of controlling the individual activity level of insulin

[0009] At present, there is no standardized immuno-mass spectrometry kit for clinical direct identification of endogenous insulin and detection of exogenous insulin in protein mass spectrometry analysis

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