Industrialized production method for anoectochilus roxburghii seedlings

A production method and technology of clematis, which are applied in the field of factory production of clematis seedlings, can solve problems such as low reproduction coefficient, high pollution rate, and long growth cycle, so as to improve the rapid propagation coefficient, relieve the increasingly depleted, cost reduction effect

Inactive Publication Date: 2013-12-25
HUZHOU ACAD OF AGRI SCI
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  • Abstract
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Problems solved by technology

Guangdong Province has also made remarkable achievements in industrial seedling cultivation, but the tissue culture seedling cultivation technology of A.
At present, the main tissue culture and rapid propagation method adopted in the industrial production of A. romanifolia is the method of inducing cluster buds. This method has a low reproduc...

Method used

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  • Industrialized production method for anoectochilus roxburghii seedlings

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Embodiment 1

[0018] A factory production method of clematis seedlings, comprising the following technical steps:

[0019] (1) In spring every year, select Aureus clematis plants with intact plants, strong growth, and excellent morphological characteristics as explants, disinfect them, and conduct tissue culture. The culture conditions are room temperature 25±1°C, humidity 60-80%, Under the light intensity of 1000luX, the light is cultivated for 6 hours a day, and the medium is MS+TDZ 0.4mg / L+NAA 0.2mg / L;

[0020] (2) After about 30 days of germination, go to

[0021] MS+S-3307 1.0mg / L+ 6-BA 2.0mg / L+ NAA 0.6mg / L+20% mashed potato protocorm proliferation medium for proliferation, after the proliferation culture, the medium nutrients have also been consumed in large quantities; When the time is right, it should be transferred to the subculture medium for differentiation culture. The culture conditions are room temperature 25±2°C, humidity 60-80%, light intensity 1000lux, light time 8 hours a...

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Abstract

The invention discloses an industrialized production method for anoectochilus roxburghii seedlings. The industrialized production method comprises the following steps of (1) selecting anoectochilus roxburghii plants which are complete plant, grow strongly and have excellent morphological characteristics as explants, carrying out disinfection treatment on the selected anoectochilus roxburghii plants and carrying out tissue culture; (2) carrying out proliferation culture and then transferring to a subcultured medium to carry out differentiation culture; (3) performing second generation proliferation; (4) carrying out rooting and growth-promoting culture; and (5) hardening the seedlings. Compared with conventional methods, the method provided by the invention can greatly reduce cost of industrialized production. A rapid propagation coefficient of the anoectochilus roxburghii seedlings is increased by 3-4 times than that cultured in a conventional tufted-bud induction manner.

Description

technical field [0001] The invention relates to an industrial production method of clematis seedlings. Background technique [0002] Golden clematis, also known as golden thread orchid and golden silk grass, is a perennial rare Chinese herbal medicine of the Orchidaceae lip plant. It is used in a wide range of folks, and is known as "King of Medicine", "Golden Grass", "God Grass", "Bird Ginseng" and other laudatory titles. It has been determined that the amino acid composition, ingredients, content and anti-aging active trace element content of Golden Silk Lotus are higher than those of domestic and wild American ginseng, and it has been used as a common herbal medicine for hundreds of years. Clematis has high requirements on the production environment, and the seeds are underdeveloped, so it is extremely difficult to germinate under natural conditions. In addition, the natural environment has been severely damaged in recent years, and people have over-harvested it all year...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G1/00
Inventor 李艳冬
Owner HUZHOU ACAD OF AGRI SCI
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