Bacillus megatherium, method for preparing microbial inoculum through solid fermentation of bacillus megatherium and application of microbial inoculum
A technology of Bacillus megaterium and solid fermentation, applied in the field of microbial preparations, can solve the problems of low capacity, restrict the effective utilization of feed resources, etc., and achieve the effects of wide application, improved feed utilization, and low cost
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Embodiment 1
[0030] Embodiment 1: Determination of enzyme production characteristics of Bacillus megaterium JSSW-JD-2F5
[0031] (1) Strain activation:
[0032] Aseptically opened the freeze-dried preserved strain of strain JSSW-JD-2F5, streaked and inoculated on the slant of LB agar test tube, cultured at 30°C for 40 hours, then transferred to the slope of LB agar eggplant bottle, and cultured at 30°C for 40 hours. Microscopic examination showed that more than 90% of the bacteria had formed spores, and mature seeds were obtained.
[0033] (2) Determination of enzyme production characteristics
[0034]Use an inoculation needle to take a small amount of bacteria in the above step (1) and place them on the starch hydrolysis medium plate, casein medium plate, cellulose medium plate and lipase medium plate, and repeat the spotting for each medium 3 times , and cultivated at 30°C for 24h. Add a small amount of Lugol's iodine solution dropwise on the cultivated starch hydrolysis medium plate,...
Embodiment 2
[0044] Embodiment 2: the preparation of bacillus megaterium solid fermentation bacterial agent
[0045] (1) Strain activation:
[0046] Aseptically opened the freeze-dried preserved strain of strain JSSW-JD-2F5, streaked and inoculated on the slant of LB agar test tube, cultured at 30°C for 40 hours, then transferred to the slope of LB agar eggplant bottle, and cultured at 30°C for 40 hours. Microscopic examination showed that more than 90% of the bacteria had formed spores, and mature seeds were obtained.
[0047] (2) Preparation of seed bacterial suspension: scrape and wash the mature seeds from the slope with sterile water, put them into a sterilized triangular flask with sterile glass beads inside, shake and disperse the bacterial sludge to make a uniform bacterial suspension ,spare.
[0048] (3) Solid fermentation culture: put the solid fermentation medium into a fermentation bottle at a volume of 10% (w / v), seal it with eight layers of gauze, and sterilize at 121°C for...
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