Washing composite enzyme containing fungus alpha-amylase and preparation method of washing composite enzyme

A technology of amylase and compound enzyme, applied in the field of washing compound enzyme, can solve the problems of poor storage stability, single function, single type of washing enzyme, etc.

Active Publication Date: 2015-04-01
湖南新鸿鹰生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The technical problem solved by the present invention is to overcome the defects of the existing washing enzymes such as single type, low enzyme activity, poor storage stability, and single function, use wood chips with dustproof, anti-cross-color and increased washing effect as the carrier, and use high-temperature-resistant fungi α-amylase is the main raw material, and scientifically compounded plant extracts with antibacterial and aroma-enhancing effects, non-ionic surfactants with diffusion and penetration effects, alkaline protease, alkaline lipase, and Alkaline cellulase, an activator that promotes the full exertion of enzyme activity, a protective agent and an antioxidant that prevent compound enzymes from being inactivated due to changes in external conditions, etc., prepare a kind of enzyme with high storage stability, high enzyme activity and efficacy, and A comprehensive detergent complex enzyme with dust-proof, anti-bacterial, anti-color and fragrance-enhancing effects

Method used

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  • Washing composite enzyme containing fungus alpha-amylase and preparation method of washing composite enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1 raw material preparation

[0056] 1. Preparation of fungal α-amylase:

[0057] The preparation method of the fungal alpha-amylase comprises the following steps:

[0058] (1) Activation of bacteria

[0059] Inoculate the well-preserved slant strain of Trichoderma reesei CCTCC NO:M 2013602 on the slant medium, culture at 30°C for 40 hours to activate the strain, and activate it 3 times;

[0060] (2) Expanded cultivation of liquid seeds

[0061] ①First-level seed culture: wash the spores of the slanted bacteria after activation in step (1) with sterile water, insert them into a 500-ml shaker flask, fill the liquid seed medium with 100 ml, and culture on a shaker at 30°C and 100rpm for 40 hours;

[0062] ②Cultivation of secondary seeds: Inoculate 10% of the primary seeds into 500 ml secondary seed shake flasks, and the cultivation conditions are the same as those of the primary seeds;

[0063] ③Third-level seed cultivation: insert the second-level seeds int...

Embodiment 2

[0081] A washing complex enzyme containing fungal α-amylase, prepared from the raw materials of the following weight components:

[0082] 50 parts of sawdust, 30 parts of fungal α-amylase, 25 parts of alkaline protease, 25 parts of alkaline lipase, 20 parts of plant extract, 6 parts of alkaline cellulase, 5 parts of nonionic surfactant, 4 parts of activator part, 2 parts of protective agent, 0.4 part of antioxidant;

[0083] The sawdust, fungal α-amylase, and plant extract are all prepared in Example 1;

[0084] The mass components of the nonionic surfactant are: 35 parts of alkyl polyglycosides (APG), 25 parts of alkyl glucamides (AGA), 10 parts of N-dodecyl ethylenediamine triacetate (ED3A) , isomerized alcohol ether carboxylate AEC-110710 parts, lauramide ether carboxylate (LAEC) 7 parts, Pingpingjia c-1251 parts, JFC 1 part;

[0085] The activator is formed by uniformly mixing inorganic salts with the following mass components: 20 parts of sodium sulfate, 12 parts of cal...

Embodiment 3

[0090] A washing complex enzyme containing fungal α-amylase, prepared from the raw materials of the following weight components:

[0091] 45 parts of sawdust, 25 parts of fungal alpha-amylase, 20 parts of alkaline protease, 20 parts of alkaline lipase, 15 parts of plant extract, 3 parts of alkaline cellulase, 2 parts of nonionic surfactant, 2 parts of activator 1 part, 1 part of protective agent, 0.3 part of antioxidant;

[0092] The sawdust, fungal α-amylase, and plant extract are all prepared in Example 1;

[0093] The mass components of the nonionic surfactant are: 30 parts of alkyl polyglycosides (APG), 20 parts of alkyl glucamides (AGA), 8 parts of N-dodecyl ethylenediamine triacetate (ED3A) , isomerized alcohol ether carboxylate AEC-11078 parts, lauramide ether carboxylate (LAEC) 5 parts;

[0094] The activator is formed by uniformly mixing inorganic salts with the following mass components: 15 parts of sodium sulfate, 10 parts of calcium chloride, 5 parts of zinc chlo...

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Abstract

The invention discloses a washing composite enzyme containing fungus alpha-amylase and a preparation method of the washing composite enzyme, and belongs to the technical field of preparation of a washing enzyme. A washing composite enzyme, which is high in storage stability, high in enzyme activity and efficacy, comprehensive in enzyme system and excellent in decontaminating effect, and has dustproof, antibacterial, cross-color resistant and essence-flavored effects is prepared from sawdust serving as a carrier and heat-resistant fungus alpha-amylase serving as a main raw material, as well as a plant extract, a nonionic surfactant, alkaline protease, alkaline lipase, alkaline glucanase, an activator, a protective agent, an antioxidant and the like which are scientifically compounded. Compared with commercially available special composite enzyme for washing, the enzymatic laundry powder produced by using the washing composite enzyme containing fungus alpha-amylase has the advantages that the detersive power is strong; the detersive power for carbon black dirt, protein dirt and sebum dirt is respectively improved by 88%, 107% and 129%; the deposition ash content ratio is reduced by 36%; the residual bacterial count on the clothes surfaces is reduced by 71%; skin and clothes are not damaged, cross-color resistant ability is high, fragrance lasts for a long time and no dust is generated in a use process.

Description

technical field [0001] The invention relates to a washing compound enzyme, in particular to a washing compound enzyme containing fungal alpha-amylase and a preparation method thereof. Background technique [0002] The detergent industry involves a wide range of areas and a large volume. The annual consumption in my country is about 3 million tons, while the annual consumption in the world is about 25 million tons. In order to improve the decontamination effect, the content of surfactant and sodium tripolyphosphate in the detergent is as high as 30%-40%. These high-phosphorus substances flow into rivers and lakes after being washed, causing over-fertilization of lakes and rivers, leading to the proliferation of harmful organisms such as microorganisms and algae, and causing water pollution. This serious phenomenon has attracted more and more people's attention and attention. Therefore, adding enzyme preparations to detergents can reduce the amount of surfactants and sodium ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11D3/386C11D3/48C11D3/60C11D1/825C11D1/83
Inventor 李洪兵张锦杰李海清胡永明朱永明辛钢
Owner 湖南新鸿鹰生物工程有限公司
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