Rapid test paper or paper card for zinc ion colloidal gold chromatography and manufacturing method

A technology for detecting test strips and zinc ions, applied in the fields of immunology and hygiene inspection, can solve the problems of long detection time, difficult adaptation and high cost, and achieve the effects of strong timeliness, easy operation and control, and mild reaction process.

Inactive Publication Date: 2013-12-25
HENAN INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional heavy metal ion detection methods include flame atomic absorption spectrometry, inductively coupled plasma mass spectrometry, flame atomic absorption spectrometry, voltammetry, ion chromatography, and electrothermal atomization atomic absorption spectrometry, etc. Analytical instruments are carried out in key laboratories, which cannot be used for on-site testing, and are limited by high costs, limited processing capacity, and long testing time, which is not conducive to popularization and application in production, and it is difficult to adapt to the environment and on-site spot checks of market products and product processing. Requirements for fast-track export clearance

Method used

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  • Rapid test paper or paper card for zinc ion colloidal gold chromatography and manufacturing method
  • Rapid test paper or paper card for zinc ion colloidal gold chromatography and manufacturing method
  • Rapid test paper or paper card for zinc ion colloidal gold chromatography and manufacturing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1. Zn 2+ Synthesis of artificial antigen

[0050] It is called 20mg klh, which is dissolved in N-(2-hydroxye ethyl) -N ’-2-ethylene sulfonic acid buffer (HBS) with a 1ml concentration of 10mmol / L, PH9.0, to form a KLH solution.

[0051] Called 10mg metal octon agent for amino benzhenyl-titthhamine and pentthylene (P-NH2-BN-DTPa), and dissolved in 1ml of two-sectaria (DMSO)The coordinated solution is added to the KLH solution of 1ml under gently stirring, and the pH to 9.0 is adjusted with a 10 mol / L NaOH solution, and the reaction of the room temperature is 24h;Ultrafilled, super filter pipes were washed 3 times with 10 mmol / L, PH9.0 HBS, and then washed 2 times with 10 mmol / L, PH7.4 HBS buffer to remove the unresponsive small molecular metal metal co -agent.

[0052] Take 16 μL concentration to 733.7mmol / L zinc solution drip to the response carrier protein solution, use NaOH solution to adjust its pH value to 9.0, room temperature shake the bed for 24 hours, and ...

Embodiment 2

[0055] Example 2. ZN 2+ Preparation of monoclonal antibodies

[0056] (1) Animal immunity.Immune BALB / C mice, use ZN 2+ -DTPA-KLH is immune to 6 weeks of age female Balb / C mice, with a dose of 20 μg / 0.2ml / piece.Shou -free, dilute Zn with sterilization PBS 2+ -DTPA-KLH, mixed with the equivalent CFA emulsification; strengthening immunity, diluted Zn with sterilization PBS 2+ -DTPA-KLH, mixed with the equivalent IFA, and emulsified for the first 3 weeks after the first free exemption. After that, it is immune once every 2 weeks.30 min, set up overnight at 4 ° C, 4000 R / min 4 ° C centrifugal 5 min, take it to clear, and save for later use.

[0057] (2) Cell fusion spare mouse selection.Indirect ELISA detection 2+ Cheese PAB cost, block ELISA detection Zn 2+ -Dtpa Pab to Zn 2+ -DTPA IC 50 , The highest selection is the highest, IC 50 The lowest mice are used for cell fusion after super exemption.

[0058] (3) Screening of positive hybrid tumor cells.Cell fusion, preheat the PEG-1500...

Embodiment 3

[0061] Example 3. Anti -Zn 2+MAB's identification

[0062] (1) Determination of ascites.Mouse injection of mice after 10 d of paradise for abdominal cavity injection cloning cell strain 10 7 For a cell, take ascites after 10 d, the saturated ammonium sulfate salt analysis method is pure, indirect ELISA measures Zn 2+ MAB's ascites are 1: 1.2 × 10 6 It

[0063] (2) Sensitivity identification.Use block ELISA to measure Zn 2+ MAB to different concentrations zn 2+ -Edta standard liquid inhibitory rate, inhibitory rate B / B 0 For vertical coordinates, use different concentrations ZN 2+ -Edta's pairs of values are horizontal coordinates, draw standard inhibitory curves, perform relevant regression analysis, calculate Zn 2+ mab to Zn 2+ -Edta IC 50 EssenceZn 2+ MAB's linear regression equation is y = -33.827 x + 99.512, R 2 = 0.9891, IC 50 It is 29.08 μg / L.

[0064] (3) Special identification.Adopting cross -reaction tests, selection of mercury, lead, cadmium, copper, chromium, cobalt, ir...

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Abstract

The invention discloses rapid test paper or a paper card for zinc ion colloidal gold chromatography and a manufacturing method. The test paper structurally comprises a supporting plate, a sample pad, a gold-labeled antibody combination pad, a coating membrane and a water absorption pad, wherein the two ends of the test paper are provided with protective membranes, the sample pad, the gold-labeled antibody combination pad, the coating membrane and the water absorption pad are sequentially bonded to the supporting plate; a monoclonal antibody or a polyclonal antibody with colloidal gold labels and capable of identifying zinc ions coats the gold-labeled antibody combination pad; the coating membrane is provided with invisible detection prints printed by a carrier protein solution coupled with the zinc ions and invisible detection prints printed by a goat anti-mouse IgG solution. The test paper or the test card for colloidal gold chromatography is high in specificity and sensitivity, convenient to use, rapid, high in timeliness, vivid, visual and accurate in result displaying and wide in application range, saves expenses, and facilitates popularization and application.

Description

Technical field [0001] The present invention involves a device for detecting trace zinc ions, especially a golden label / card and preparation method for fast detection of zinc ions, which belongs to the field of immunology and sanitary inspection. Background technique [0002] Heavy metals in soil and agricultural products are closely related to human health.Heavy metal pollution mainly refers to the biological toxicity of mercury, chromium, cadmium, lead, and metal arsenic, and also includes toxic heavy metal copper, cobalt, nickel, tin and other metal pollutants.Different metal pollution is different from other types of pollution, and has the characteristics of concealment, long -term and irreversible.With the expansion of cities and the development of large -scale industries, a large number of heavy metals enter the environment, even if the concentration is low, it may cause harm. It will eventually threaten human health through drinking water or biomass and food chains. [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/532
Inventor 王自良王爱萍范国英张海棠葛亚明王顺岗李艺丁函
Owner HENAN INST OF SCI & TECH
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