Spontaneous-luminescencebased method for evaluating drought resistance of corn seed in germination period

A spontaneous luminescence and evaluation method technology, applied in the agricultural field, can solve the problems of inaccurate destructive measurement and evaluation, long detection method cycle, time-consuming and laborious, etc., and achieve the effect of drought resistance, non-destructive, rapid and reliable evaluation effect

Active Publication Date: 2014-01-01
盐城市双新建设投资有限公司
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Problems solved by technology

[0008] The purpose of the present invention is to provide a method for evaluating drought resistance of corn seeds based on spontaneous luminescence, which solves the problems of long cycle, time-consuming and laborious, destructive measurement and evaluation of existing detection methods

Method used

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  • Spontaneous-luminescencebased method for evaluating drought resistance of corn seed in germination period
  • Spontaneous-luminescencebased method for evaluating drought resistance of corn seed in germination period
  • Spontaneous-luminescencebased method for evaluating drought resistance of corn seed in germination period

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Embodiment

[0036] To judge the drought resistance of Wanrui 168 and Yandan 8 maize seeds during germination, plump seeds with the same size and appearance were selected, washed with distilled water, and added with a mass fraction of 0.2% HgCl 2 After disinfection and cleaning, place them evenly in petri dishes covered with two layers of filter paper, place 120 corn seeds in each petri dish, add PEG-6000 solution with an osmotic potential of -0.3MPa, keep the temperature at 25°C and avoid light For cultivation, the amount of PEG-6000 solution added is subject to not submerging the seeds. Considering the effect of seed water absorption on osmotic potential, the PEG-6000 solution was replaced every 24 hours. Another group of seeds was taken as a control, and the conditions of the control group were the same as those of the treatment group except that they were cultured with distilled water. There were 4 replicates in each treatment group and control group.

[0037] Record the germination ...

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Abstract

The invention discloses a spontaneous-luminescence-based method for evaluating the drought resistance of corn seeds in a germination period. The method comprises the following steps: carrying out water stress on germinated corn seeds by adopting a PEG-6000 solution at -0.3MPa; measuring the spontaneous luminescence of the germinated corn seeds every 24 hours in the germination process; evaluating the magnitude of the drought resistance of the corn seeds in the germination period through the magnitude of the relative change rate of the spontaneous luminescence after the corn seeds are germinated under the water stress for 24 hours. According to the spontaneous-luminescence-based method for evaluating the drought resistance of the corn seeds in the germination period, which is disclosed by the invention, only the water stress is required to be carried out on the germinated corn seeds without carrying out a field test, the method can be used for directly measuring the spontaneous luminescence of the corn seeds in the germination process, and realizing the lossless and the fast evaluation of the magnitude of the drought resistance of the corn seeds in the germination period through the magnitude of the relative change rate of the spontaneous luminescence and is simple, practical and reliable in evaluation effect.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and relates to a method for evaluating the stress resistance of crops, in particular to a method for evaluating the drought resistance of corn seeds in germination stage based on spontaneous luminescence. Background technique [0002] In the arid and semi-arid areas of China, corn often encounters drought during the germination period after sowing, and the germinated corn seeds are very sensitive to drought, which often greatly affects seed germination and seedling emergence. Corn with strong drought resistance in the germination period is used Seeds are of great significance to food production in arid and semi-arid regions of my country. [0003] In order to breed maize seeds with strong drought resistance during germination, it is necessary to establish a set of methods and techniques that can accurately identify and evaluate the drought resistance of maize seeds during germination. [0004...

Claims

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Application Information

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IPC IPC(8): A01C1/00
Inventor 习岗刘锴高宇樊琳琳梁成赵燕燕
Owner 盐城市双新建设投资有限公司
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