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Injectable alginate-based biomaterial for adjuvant therapy of heart failure and preparation method thereof

A biomaterial and adjuvant therapy technology, applied in medical science, prosthesis and other directions, can solve the problems of disturbing the normal signal transduction of myocardium, triggering immune response, and no marketed products, etc., achieving simple and controllable process methods, good hydrophilicity, The effect of reducing security

Active Publication Date: 2015-12-02
奚廷斐
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be inferred that medical alginate-based biomaterials, as a new strategy for the treatment of heart failure, are mainly based on their mechanical space support function to adjust the spatial structure of the damaged myocardium, thereby reducing / blocking the deterioration of myocardial pathological remodeling, It has nothing to do with the pharmacology, immunology or biological activity of alginate-based biomaterials. At present, this strategy has made important progress in animal experiments, but it is still in the research stage, and there are no relevant marketed products at home and abroad.
[0006] In summary, it has been confirmed in the above-mentioned multiple studies that alginic acid or gels prepared with alginic acid, collagen, gelatin, etc. have a certain therapeutic effect on animal models of myocardial infarction, but there are still few reports on the application in the treatment of heart failure. , the alginic acid hydrogel prepared by the above common method will still be absorbed after staying in the body for a period of time, its tissue support and filling effect still need to be strengthened, and there is still a high probability of causing arrhythmia after injection into myocardial tissue, and it has certain Hidden dangers; introducing collagen, gelatin and other substances into the alginic acid hydrogel system can partially improve the structure and function, but due to the biological activity and immunogenicity of collagen and its degradation products such as polypeptides, it still triggers immune responses and the risk of interfering with the normal signal conduction of the myocardium, which is also confirmed by the increased proportion of cardiac arrhythmia in animal model experiments

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  • Injectable alginate-based biomaterial for adjuvant therapy of heart failure and preparation method thereof
  • Injectable alginate-based biomaterial for adjuvant therapy of heart failure and preparation method thereof
  • Injectable alginate-based biomaterial for adjuvant therapy of heart failure and preparation method thereof

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Embodiment 1

[0050] The preparation method of the injectable alginic acid-based biomaterial used for adjuvant treatment of heart failure of the present invention is as follows.

[0051] Step 1: Preparation of sodium alginate system: under sterile conditions, dissolve sterile pyrogen-free sodium alginate raw material with a weight-average molecular weight of 180,000 Da in water for injection-mannitol solution, fully dissolve at room temperature, Prepare a solution with a concentration of 2.0%.

[0052] Step 2: Preparation of the cross-linking system: under sterile conditions, disperse the aseptic and non-pyrogenic formaldehyde-calcium alginate complex system in the water for injection-mannitol solution, with a final concentration of 2.0% , wherein the solid particle size is 70-200μm.

[0053] Step 3: Under sterile conditions, fill the prepared sodium alginate system and cross-linking system into 3ml sterile syringes respectively, mix them through a three-way needle and gradually drop them ...

Embodiment 2

[0056] The preparation method of the injectable alginic acid-based biomaterial used for adjuvant treatment of heart failure of the present invention is as follows.

[0057] The first step: with embodiment 1.

[0058] Step 2: Preparation of the cross-linking system: under sterile conditions, disperse the aseptic and non-pyrogenic formaldehyde-calcium alginate complex system in the water for injection-mannitol solution, with a final concentration of 3.0% , wherein the solid particle size is 70-200μm.

[0059] The third step: with embodiment 1.

[0060] Step 4: Under sterile conditions, equilibrate at room temperature for 6 minutes to form a stable gel.

Embodiment 3

[0062] The preparation method of the injectable alginic acid-based biomaterial used for adjuvant treatment of heart failure of the present invention is as follows.

[0063] The first step: with embodiment 1.

[0064] The second step: preparation of cross-linking system: under aseptic conditions, disperse sterile pyrogen-free calcium alginate in water for injection-mannitol solution, the final concentration is 4.0%, and the solid particle size is 80- 150 μm.

[0065] Step 3: Under sterile conditions, fill the prepared sodium alginate system and cross-linking system into 3ml sterile syringes respectively, mix them through a three-way needle and gradually drop them into a sterile culture plate, mix well The process is uniform and slow; the ratio of sodium alginate system and cross-linking system: 1:1.

[0066] Step 4: Under sterile conditions, equilibrate at room temperature for 3 minutes to form a stable gel.

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Abstract

The invention relates to an injectable type alginic acid radical biological material for heart failure adjuvant therapy and a preparation method thereof. The preparation method comprises steps of: preparing two systems of a sodium alginate system and a cross-linking agent system, mixing the two components uniformly by utilizing a three-way syringe and preparing the injectable type alginic acid radical biological material for heart failure / myocardial infarction adjuvant therapy after a proper time of balancing. The materials and processing technologies which are adopted by the invention do not involve organic reagents or organic chemical reactions, the reaction systems are moderate, the conditions are controllable, and the problem of residues of toxic cross-linking agents or auxiliaries cannot be caused; a hydrogel which is prepared according to the technical scheme is injected into the spherical expanding myocardium after heart failure by using the syringe No.271 / 2, so that cardiac ventricles can be helped to reshape, the sizes of the cardiac ventricles can be effectively reduced, and the tension of ventricle walls is decreased; and the biological material can be used for heart failure adjuvant therapy, has good mechanical properties and biological properties as well as good cytocompatibility and is convenient and reliable to operate.

Description

technical field [0001] The invention relates to a biological material for adjuvant treatment of heart failure, in particular to an injectable alginic acid-based biological material for adjuvant treatment of heart failure and a preparation method thereof. Background technique [0002] Heart failure, also known as cardiac insufficiency, refers to a series of symptoms and signs that occur when the heart cannot discharge enough blood under the condition of adequate venous return, resulting in insufficient perfusion of surrounding tissues and venous congestion in the pulmonary circulation and systemic circulation, heart valve disease, coronary arteriosclerosis , hypertension, endocrine disorders, bacterial toxins, acute pulmonary infarction, emphysema or other chronic lung diseases can cause heart disease and induce heart failure. The initial myocardial injury caused by the above-mentioned various inducements can cause myocardial infarction, left ventricular hypertrophy and remod...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/20A61L27/52C08J3/24C08J3/075C08L5/04
Inventor 奚廷斐位晓娟顾其胜
Owner 奚廷斐
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