Method for step by step extracting avermectin B1a and B2a from mycelium

A technology of abamectin and mycelium, which is applied in the field of step-by-step extraction of B1a and B2a components, can solve the problems of separation and inability to separate, and achieve less impurities, high extraction rate and high crystallization yield Effect

Inactive Publication Date: 2014-01-01
王玉万 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The common problem that above-mentioned technique exists is all can not be B 1a and B 2a Completely disassembled, in addition to containing B in the mother liquor 2a , still contains about 15% B 1a unable to communicate with B 2a split apart
Therefore, using existing methods to extract B 1a , and its yield is about 75%

Method used

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  • Method for step by step extracting avermectin B1a and B2a from mycelium

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0010] Example 1, different moisture content to B 1a and B 2a Effect of Extraction Rate

[0011] The abamectin mycelium powder used in the experiment was provided by Zhejiang Shenghua Baike Co., Ltd.: the water content in the mycelium was 13-16%, B 1a Content is 14%, B 2a The content is 12%; the experiment is divided into 7 groups, respectively numbered NO.1~NO.7, one bottle for each group, and 7 parts of mycelium powder are accurately weighed, each 10.0g, and the 250ml of each group is filled with a triangle bottle, and then add 100 mL of methanol or water / methanol co-solvent to each bottle separately, as follows:

[0012] NO.1 Add 100ml of methanol with a purity of 99.5%

[0013] NO.2 Add 100ml water / methanol (2:8, volume ratio)

[0014] NO.3 Add 100ml water / methanol (3:7, volume ratio)

[0015] NO.4 Add 100ml water / methanol (4:6, volume ratio)

[0016] NO.5 Add 100ml water / methanol (4.5:5.5, volume ratio)

[0017] NO.6 Add 100ml water / methanol (5:5, volume ratio)

...

example 2

[0029] Example 2, the influence of mycelia water content on the extraction effect

[0030] Three kinds of mycelia with different water contents were used in the experiment to extract B with methanol / water co-solvent 2a , the consumption of methanol / water co-solvent is 10 times of mycelium weight (on a dry basis), and the leaching time is 3 hours, and the experimental results are shown in the following table.

[0031]

[0032] Note: The extract refers to the mixed system composed of mycelium, water and methanol.

[0033] From the above experimental results, it can be seen that extracting B with about 50% water content mycelium 2a suitable.

example 3

[0034] Example 3, extract B from the mycelium filter cake of water content 45~50% 1a and B 2a

[0035] Take 100g water content is 45 ~ 50%, containing B 1a 6.12%, B 2a Add 1 liter of methanol / water (52 / 48) to the 4.9% mycelium filter cake, stir and extract for 2 hours, and then filter with suction to obtain the first extraction filtrate and fungus residue A.

[0036] HPLC analysis: B in the first extraction filtrate 2a The concentration is 0.51%, the filtrate contains B 2a The total amount is 3.92g, B 1a The concentration is 0.016%, the filtrate contains B 1a The total amount is 0.126 g.

[0037] The fungus residue A was extracted twice with 10 times the volume of methanol / water (50 / 50), and suction filtered to obtain the second extraction filtrate and the fungus residue B.

[0038] HPLC analysis: B in the second extraction filtrate 2a The concentration is 0.12%, the total amount is 1.02g, B 1a The concentration is 0.0192%, and the total amount is 0.1536g.

[0039]...

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Abstract

The invention discloses a method for selectively extracting avermectin B1a and B2a from streptomyces avermitilis mycelium, and the method is prominently characterized in that the avermectin B1a and B2a are extracted from the mycelium in two steps, first water / methanol or water / ethanol cosolvent is used for selective extraction of the B2a from the mycelium, and then an organic dissolvent such as methanol or ethanol and the like is used for extraction of the B1a from the mycelium. The method can effectively separate the B1a and the B2a, facilitates subsequent separation and purification of the B1a and the B2a, and can enhance the crystallization yield of the B1a and the B2a.

Description

technical field [0001] The present invention relates to abamectin B 1a and B 2a The extraction method, specifically involves the step-by-step extraction of B from mycelium 1a and B 2a Components, using this extraction method, can make B 1a with B 2a effective separation. Background technique [0002] Abamectin is an agricultural antibiotic produced by the fermentation of Streptomyces avermanii. It is a group of sixteen-membered macrolide compounds, including avermectin A 1a / A 1b (A 1 ), A 2a / A 2b (A 2 ), B 1a / B 1b (B 1 ), B 2a / B 2b (B 2 ), where Abamectin B 1 It has been commercialized for the control of animal parasitic diseases and crop pests, and other components have not yet been developed and applied. In 1993, my country successfully developed Abamectin B commercially 1 , the fermentation process of the production strain at that time produced A component (A 1 and A 2 ) and B component (B 1 and B 2 ), since 2009, domestic manufacturers began t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/08C07H1/08
Inventor 王玉万潘贞德戴晓羲
Owner 王玉万
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