Nucleic acid extraction chip capable of extracting nucleic acid of gram-positive bacteria and gram-negative bacteria
A Gram-positive bacteria and Gram-negative technology, applied in biochemical equipment and methods, specific-purpose bioreactors/fermenters, biochemical instruments, etc., can solve the problems of unfavorable nucleic acid miniaturization and automation, and achieve Facilitate automatic operation, simple operation, and promote the effect of development
Inactive Publication Date: 2015-04-29
SCI RES TRAINING CENT FOR CHINESE ASTRONAUTS
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Problems solved by technology
However, most of them involve centrifugation, which is not conducive to miniaturization and automation of nucleic acid extraction
Method used
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[0017] Such as figure 1 As shown, the nucleic acid extraction chip for extracting gram-positive bacteria and gram-negative bacteria nucleic acid of the present invention includes a control channel layer, an elastic film layer, and a liquid channel layer. The lower surface of the control channel layer is tight with the upper surface of the elastic film layer. The lower surface of the elastic film layer and the upper surface of the liquid channel layer are arranged in close contact with each other. The left side of the upper surface of the liquid channel layer is provided with a nucleic acid extraction liquid channel 6, a rinsing liquid channel 5, Enter EDTA SDS channel 4, enter nuclease channel 3, enter cell lysate channel 2 and enter cell suspension channel 1, enter nucleic acid extract channel 6, enter rinse solution channel 5, enter EDTA SDS channel 4, enter nuclease channel 3 , The left ends of the cell lysate channel 2 and the cell suspension channel 1 are respectively prov...
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Abstract
The invention discloses a nucleic acid extraction chip capable of extracting nucleic acid of gram-positive bacteria and gram-negative bacteria. The nucleic acid extraction chip comprises a control channel layer, an elastic thin film layer and a liquid channel layer; the upper surface of the liquid channel layer is provided with a nucleic acid extracting solution inlet channel (6), a rinsing solution inlet channel (5), an EDTA SDS inlet channel (4), a nuclease inlet channel (3), a cell lysis solution inlet channel (2) and a cell suspension inlet channel (1); the left ends of the nucleic acid extracting solution inlet channel (6), the rinsing solution inlet channel (5), the EDTA SDS inlet channel (4), the nuclease inlet channel (3), the cell lysis solution inlet channel (2) and the cell suspension inlet channel (1) are respectively provided with a liquid inlet which penetrates the liquid channel layer downward. The nucleic acid extraction chip capable of extracting nucleic acid of gram-positive bacteria and gram-negative bacteria is based on silicon bead test, centrifugation treatment is not needed, the nucleic acid extraction chip is convenient for automation operation, operation is simple and rapid, detection is fast, and detection effect is objective.
Description
Technical field [0001] The invention relates to a nucleic acid extraction chip for extracting nucleic acids of gram-positive bacteria and gram-negative bacteria. Background technique [0002] The extraction of nucleic acid is a basic technology involving biological manipulation. So far, people have developed a variety of methods to extract nucleic acids. However, most of them involve centrifugation, which is not conducive to miniaturization and automation of nucleic acid extraction. Summary of the invention [0003] The purpose of the present invention is to provide a silicon-based bead that does not require centrifugal treatment, is convenient for automatic operation, simple and convenient for operation, rapid detection, and objective, and can provide technical support for scientific research, disease diagnosis, genetic testing, forensic identification, etc. Nucleic acid extraction chip for extracting nucleic acid from Gram-positive bacteria and Gram-negative bacteria. [0004] ...
Claims
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IPC IPC(8): C12M1/00
CPCC12N15/1003
Inventor 顾寅郑文富谭映军蒋兴宇李莹辉
Owner SCI RES TRAINING CENT FOR CHINESE ASTRONAUTS