Mycopasma hyopneumoniae strain

A technology of Mycoplasma hyopneumoniae and strains, applied in the directions of bacteria, microorganisms, biochemical equipment and methods, etc., can solve the problems of high nutritional requirements, harsh growth conditions, difficult separation and screening of Mycoplasma hyopneumoniae, etc., and achieves good immunogenicity, The effect of reducing production costs

Inactive Publication Date: 2014-01-01
HEILONGJIANG UNIV
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Problems solved by technology

The in vitro culture of Mycoplasma hyopneumoniae requires harsh growth conditions, high nutritional requirements and slow growth rate. During the isolation

Method used

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  • Mycopasma hyopneumoniae strain

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specific Embodiment approach 1

[0011] Specific embodiment one: In this embodiment, a Mycoplasma hyopneumoniae bacterial strain has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, the preservation number is CGMCC No.8096, the preservation date is August 15, 2013, and the preservation address is No. 3, No. 1 Courtyard, Beichen Road, Chaoyang District, Beijing, it was named Mycopasma hyopneumoniae (Mycopasma hyopneumoniae) HDZK-Mhp57.

[0012] In the present embodiment, a Mycoplasma hyopneumoniae bacterial strain, its isolation process is as follows:

[0013] 1. Take the lungs affected by mycoplasma pneumonia, cut the edge tissue of the diseased part with sterile surgical scissors in the ultra-clean bench, put it into a sterile plate, cut the diseased lung tissue into small pieces, inoculate Friis broth, and culture at 37°C until cultured liquid discoloration;

[0014] 2. After the color of the culture medium changes, take 0.5 mL of the cult...

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Abstract

The invention discloses a mycoplasma hyopneumoniae strain which is preserved in the Ordinary Microorganism Center of the Chinese Microorganism Culture Collection and Management Committee which is located in #3, No.1 Yard, Beichen Road, Chaoyang District, Beijing, the preservation number is CGMCC No.8096, and the preservation date is August 15th, 2013. The name of the strain is mycoplasma hyopneumoniae HDZK-Mhp57. According to the mycoplasma hyopneumoniae HDZK-Mhp57, the viable bacteria titer of a prepared acellular culture medium is as high as 1012CCU/mL, the immunogenicity is good, the pneumonia lesion is reduced by more than 90%, the generation cost of an animal biological product is greatly lowered, the stain can be prepared into preventing animal biological products or veterinary medicines, and a foundation for researching and preparing mycoplasma hyopneumoniae inactivated vaccines, genetic engineering vaccines and mycoplasma hyopneumoniae diagnosis kits is laid.

Description

technical field [0001] The invention relates to a Mycoplasma hyopneumoniae bacterial strain. Background technique [0002] Mycoplasma pneumonia of swine (MPS), also known as swine asthma or porcine endemic pneumonia, MPS is a chronic, contact, non-fatal respiratory disease with a domestic incidence of over 80%. The disease can affect the production performance, weight gain and feed remuneration of pigs, and can be secondary to other infectious diseases. It is one of the infectious diseases that the pig industry focuses on control. The pathogen of swine mycoplasma pneumonia is Mycopasma hyopneumoniae (Mhp), which can be transmitted through direct contact between sick pigs and healthy pigs or through aerosols. The general transmission distance can reach 4.7km, and the latest research shows that its transmission distance can be as far as 9.2km , therefore, Mhp is extremely difficult to prevent and control. Pigs infected with Mhp will have dyspnea, body weight loss, and immuno...

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Application Information

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IPC IPC(8): C12N1/20C12R1/35
Inventor 赵凯刘洪斌张凤强毛春玲
Owner HEILONGJIANG UNIV
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