Method for extracting 10-DAB from taxus chinensis according to continuous leaching adsorption enrichment method

Abstract

The invention relates to a method for extracting 10-DAB from taxus chinensis according to a continuous leaching adsorption enrichment method. According to the method, the leaching extraction technology and the macroporous resin adsorption technology are combined; the method comprises the following steps: dissolving 10-DAB-related components in taxus chinensis leaves into water through the leaching with weak acidic water, obtaining an oily product after the completion of the resin enrichment and the hydrazinolysis, adding acetonitrile, stirring, and precipitating a solid to obtain a crude 10-DAB product; using a resin chromatographic column to perform purification and recrystallization on the crude 10-DAB product to obtain a pure 10-DAB product with the purity of higher than 98%. Through the adoption of the method, the 10-DAB extraction rate from high-10-DAB-content leaves can be higher than 90%, the 10-DAB extraction rate from low-10-DAB-content leaves can be higher than 75%,and with the 10-DAB converted from the hydrazinolysis, the 10-DAB extraction rate reaches 150-300%; the method has relatively low requirements on equipment, can reduce discharge of an organic solvent, is small in investment, enables the 10-DAB productivity of dried taxus chinensis leaves with relatively low 10-DAB content to reach 0.08-0.15% that can not be obtained by adopting the traditional technology.

Description

technical field [0001] The invention relates to a method for extracting organic matter from plants, in particular to a method for extracting 10-DAB from yew by a continuous immersion adsorption enrichment method. Background technique [0002] Taxanes are clinically important anticancer drugs. At the end of 1992, paclitaxel produced by Bristol-Myers Squibb Company of the United States was approved for marketing. In 1994, the sales of paclitaxel reached 400 million U.S. dollars, and in 2000, the sales reached 1.6 billion U.S. dollars. In 2003, the total sales of paclitaxel in the world exceeded 2 billion U.S. dollars. In recent years, sales have been increasing at a rate of 20% per year . [0003] Docetaxel, also known as docetaxel, belongs to taxane compounds. It was launched in Mexico in April 1995, and then it was launched in Britain, the United States, France, Italy, Germany, Japan and other countries. The annual sales volume is year by year In 2000, the sales revenue ex...

AI Technical Summary

Problems solved by technology

However, due to species, climate and other reasons, whether it is Taxus chinensis or Taxus Mandia, the contents of paclitaxel and 10-DAB in branches and leaves are very low, and 10-DAB can only reach below 0.5‰. Existing 10-DAB extraction technology It cannot be processed, and the cost cannot be controlled by traditional methanol immersion or our newer ultrasonic water extraction technology in the early stage

Many planting bases can only sell yew trees as gardening and greening trees, and cannot realize their maximum medicinal value

Images