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Application of mango crude extracts to preparation of drugs and food for controlling cardiovascular diseases

A technology of mango, disease, applied in the field of medicine to solve problems such as less attention

Active Publication Date: 2014-01-22
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, less attention has been paid to the richness of pentagallotannins in mango pulp and kernels and the richness of mangiferin in mango peel

Method used

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  • Application of mango crude extracts to preparation of drugs and food for controlling cardiovascular diseases
  • Application of mango crude extracts to preparation of drugs and food for controlling cardiovascular diseases
  • Application of mango crude extracts to preparation of drugs and food for controlling cardiovascular diseases

Examples

Experimental program
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Effect test

Embodiment 1

[0024] In the present invention, the main component is the mango seed kernel and pulp crude extract of gallic tannin. The operation method is carried out according to the following two steps:

[0025] (1) Enrichment of gallic tannins from mango by-products: 1.5 g of Marchesu (MQS), Tainong No. 1 (TN1), Zihuamang (ZHM) fruit kernels and peel freeze-dried powder were weighed respectively, and 80% Acetone (solid-to-liquid ratio 1:20) ultrasonically extracted twice for 30 minutes, centrifuged at 8000 rpm for 10 minutes, combined the two supernatants, evaporated to dryness on a rotary evaporator to obtain extract, dissolved in 25 ml ddH 2 O middle;

[0026] (2) C18 solid-phase extraction column (Waters 12cc, 2 g) was first activated with 15 ml of methanol, then equilibrated with 15 ml of water, and then loaded with 25 ml of the extract, after fully adsorbed, rinsed with 25 ml of water to remove Water-soluble impurities such as sugar and acid were then eluted with 25 ml of methanol...

Embodiment 2

[0029] In the present invention, the main component is the mango peel crude extract operating method of mangiferin according to the following steps:

[0030] (1) Weigh 10 g of mango peel, add 200 ml of extract solution (methanol: ethanol: acetone 1:1:1, v / v / v), soak for 2 h, ultrasonicate for 30 min, filter to get supernatant, and add 200 ml of filter residue Ultrasonicate 30 min of ml extract, filter, combine the two supernatants, 45 ℃ rotary evaporation to extract, double distilled water to dissolve the extract to obtain 210 ml of crude extract;

[0031] (2) 210 ml of the crude extract was extracted twice with equal volume of ethyl acetate, the ethyl acetate phase was concentrated and then extracted with water once, and the lower phase was combined and rotatated at 45°C to remove the ethyl acetate to obtain 207 ml of the aqueous phase extract;

[0032] (3) 207 ml, inject HPD100 macroporous resin (20 ml), flow rate 2 ml / min, let stand for 1 hour after injection, wash 10 BV wi...

Embodiment 3

[0036] Protective effect of crude extract of mango peel on human umbilical vein endothelial cells (HUVEC). The specific steps are as follows: HUVEC cells in the logarithmic growth phase were digested with trypsin, pipetted into a single-cell suspension, and mixed with 7×10 3 Cells / well were inoculated in 96-well culture plate, 100 microliters of culture medium per well, cultured overnight in a 37°C, 5% CO2 incubator; cells adhered to the wall, and fruit extracts of appropriate concentration (5, 10, 20 μg / mL ) for 4 hours or the positive drug NAC for 4 hours, then add 0.5mM hydrogen peroxide; after 24 hours, fix with 10% trichloroacetic acid for 1 hour; wash with double distilled water, after drying, add 100 microliters to each well SRB solution (4 mg / mL), stained at room temperature for 20 minutes, washed with 1% acetic acid, and dried; 100 microliters of 10 mM Tris solution was added to each well to dissolve SRB; the OD value of each well was detected by a microplate reader (...

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Abstract

The invention provides an application of mango extracts to preparation of drugs or health care products for controlling tumor diseases. The mango extracts are crude pure powder obtained by extracting mango peels or kernels with 80% of acetone and carrying out primary purification on the extracts through C18 solid phase extraction. The main ingredients of the mango extracts include fivefold gallotannin and derivatives thereof. The drugs or the health care products are prepared from the mango extracts and preparation allowable excipients or food additives. Researches prove that the mango extracts have the activity of inhibiting breast cancers, liver cancers and leukemia and can be used for preparing the drugs for treating breast cancers, liver cancers and leukemia and inhibiting cell activities of breast cancers, liver cancers and leukemia or can be developed into functional food or health care products. The invention provides a new application of mango processing byproducts, namely the mango peel and kernel extracts to tumor treatment drugs or health care products.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to the protective effect of mango crude extract from natural sources on vascular endothelial cell damage caused by oxidative stress, in particular to a kind of mango crude extract used in the preparation of drugs for preventing cardiovascular diseases, functional food or health products in the application. Background technique [0002] Mango is a Anacardiaceae mango ( Mangifera indica ) fruit, rich in nutrients. Studies in recent years have shown that mango contains active substances such as carotenoids, flavonoids and their derivatives, and phenolic acids, which have anti-oxidation, anti-inflammatory, antibacterial, immune regulation, anti-tumor and hypoglycemic activities, and are favored by people. extensive attention. However, less attention has been paid to the richness of pentagallotannins in mango pulp and kernels and the richness of mangiferin in mango peel. [0003] The changes in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/22A23L1/30A23L1/212A61P9/00A61P9/10A61P3/06A23L33/105
Inventor 杨波何俏军丁玲陈昆松李鲜孙崇德
Owner ZHEJIANG UNIV
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