Separating culture medium of strains of wild lactarius deliciosus and cultivation and acclimation method

A strain separation and culture medium technology, applied in the biological field, can solve the problems that wild resources are difficult to meet market needs, and achieve the effect of simple operation method and reduced production cost

Inactive Publication Date: 2014-01-29
GUIZHOU INST OF BIOLOGY
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Problems solved by technology

[0002] Pine Milk Mushroom ( Lactarius deliciosus ) belongs to the genus Russulaceae and the genus Lactarius, also known as delicious pine milk mushroom, pine tree mushroom, and pine mushroom. It is a rare and precious natural edible fungus. The price in the domestic and foreign markets is relatively high, but it is difficult to meet the market demand only by wild resources

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  • Separating culture medium of strains of wild lactarius deliciosus and cultivation and acclimation method

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Embodiment Construction

[0008] Embodiments of the present invention: wild pine milk mushroom bacterial classification separation culture medium, calculates by weight, gets pine needle 30g, KH 2 SO 4 0.3g, MgSO 4 0.1g, 200g of potato, 20g of glucose, 20g of agar and 0.5g of peptone, add water to make up to 1000ml, then heat to boiling, filter with 4 layers of gauze, divide into test tubes, and make slant culture medium at 121 Under the conditions of ℃ and 0.15MPa, sterilize for 25 minutes to obtain the isolation medium of wild Lactobacillus pine mushroom strains.

[0009] Cultivation and acclimation method of wild milk mushroom strain isolation medium: 1) Select fresh wild milk mushroom strains with good shape and no discoloration; perform tissue separation operation in the inoculation box, and take them from the intersection of the cap and the stipe A piece of tissue is surface-sterilized with alcohol with a concentration of 75% by mass, then picked into the inclined surface of the test tube wit...

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Abstract

The invention discloses a separating culture medium of strains of wild lactarius deliciosus and a cultivation and acclimation method. The separating culture medium and the cultivation and acclimation method have the advantages that components such as pine needle and potatoes are comprehensively proportioned, and the purpose of separating the strains of the lactarius deliciosus can be well achieved by the obtained culture medium; the components such as forage, cotton seed hull, rice bran, acorn bark, wheat bran and lime are also adopted as the cultivation and acclimation culture medium, the formula of the culture medium in the prior art is optimized, and good effect for acclimation culture can be achieved; and due to the culture medium for separating the strains of the lactarius deliciosus and the cultivation and acclimation method, an effective and feasible method is provided for artificially acclimating the wild lactarius deliciosus. The ingredients of the culture medium are common materials, so that the production cost can be reduced; the operation method is simple and easy to operate, and by virtue of the cultivation and acclimation method provided by the invention, the basis can be provided for large-scale production.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a culture medium for the separation of wild lactobacillus pine mushroom strains and a method for cultivating and domesticating them. Background technique [0002] Pine Milk Mushroom ( Lactarius deliciosus ) belongs to the genus Russulaceae and the genus Lactarius, also known as delicious pine milk mushroom, pine tree mushroom, and pine mushroom. It is a rare and precious natural edible fungus. The prices in domestic and foreign markets are relatively high, but wild resources alone are difficult to meet market needs. Pine milk mushrooms are mycorrhizal fungi, so it is difficult to form fruiting bodies under artificial cultivation. In the formula of the strain separation culture medium of pine milk mushroom of existing report, mostly adopt pine needle liquid, humus liquid etc. as main component. Contents of the invention [0003] The object of the present invention is: provide a k...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王莹向准李巧进王姝贺红早任春光孙超
Owner GUIZHOU INST OF BIOLOGY
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