Separating culture medium of strains of wild lactarius deliciosus and cultivation and acclimation method
A strain separation and culture medium technology, applied in the biological field, can solve the problems that wild resources are difficult to meet market needs, and achieve the effect of simple operation method and reduced production cost
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[0008] Embodiments of the present invention: wild pine milk mushroom bacterial classification separation culture medium, calculates by weight, gets pine needle 30g, KH 2 SO 4 0.3g, MgSO 4 0.1g, 200g of potato, 20g of glucose, 20g of agar and 0.5g of peptone, add water to make up to 1000ml, then heat to boiling, filter with 4 layers of gauze, divide into test tubes, and make slant culture medium at 121 Under the conditions of ℃ and 0.15MPa, sterilize for 25 minutes to obtain the isolation medium of wild Lactobacillus pine mushroom strains.
[0009] Cultivation and acclimation method of wild milk mushroom strain isolation medium: 1) Select fresh wild milk mushroom strains with good shape and no discoloration; perform tissue separation operation in the inoculation box, and take them from the intersection of the cap and the stipe A piece of tissue is surface-sterilized with alcohol with a concentration of 75% by mass, then picked into the inclined surface of the test tube wit...
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