Rapid and quantitative detection device and method for simultaneously detecting heart-type fatty acid-binding protein and cardiac troponin I

A technology of fatty acid binding and troponin, which is applied in the field of medical clinical diagnosis, can solve the problem of not having a rapid quantitative detection device for detecting heart-type fatty acid binding protein and troponin at the same time, and achieve the effect of rapid response, accurate results and easy operation

Inactive Publication Date: 2014-01-29
天津中新科炬生物制药股份有限公司
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Problems solved by technology

At present, there is no report on the rapid quantitative detection device for simultaneous detection of heart-type fatty acid binding protein and troponin I

Method used

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  • Rapid and quantitative detection device and method for simultaneously detecting heart-type fatty acid-binding protein and cardiac troponin I
  • Rapid and quantitative detection device and method for simultaneously detecting heart-type fatty acid-binding protein and cardiac troponin I

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Embodiment

[0019] Example: Preparation and application of a rapid quantitative detection device for simultaneous detection of FABP and CTNI

[0020] 1 main material

[0021] 1.1 Biological raw material FABP and CTNI paired antibody, purchased from Finland HYTEST company; mouse anti-human IgG antibody, goat anti-mouse IgG: self-made; chloroauric acid: product of Sigma company; NC membrane: product of Sartorius company; hydrolyzed casein, polyethylene glycol Alcohol PEG20000: product of Sigma. Other commonly used reagents are analytical reagents.

[0022] 1.2 The company obtained 120 clinical samples in related hospitals, including 60 serum samples from patients diagnosed with acute myocardial infarction, with FABP detection values ​​ranging from 0.6-160ng / mL and CTNI detection values ​​ranging from 0.1-80ng / mL. 60 normal human serum samples were not detected for FABP and CTNI.

[0023] 1.3 Duplex clamping shell: designed by our company, and related companies produce and provide accordi...

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Abstract

The invention provides a rapid and quantitative detection device and a method for simultaneously detecting heart-type fatty acid-binding protein and cardiac troponin I. The device is characterized by being composed of detection test paper of the heart-type fatty acid-binding protein (FABP) and the cardiac troponin I (CTN1), a double-united clamping shell and an immunochromatography result judging and reading recorder which is internally provided with a corresponding standard curve and result judgment. The immunochromatography result judging and reading recorder is an optical detection system and is used for quantitatively judging a detection result; detection ranges to the FABP and the CTN1 are 5ng/mL-200ng/mL and 1ng/mL-100ng/mL. The detection is finished within 15-20 minutes. The rapid and quantitative detection device and the method have the advantages of united detection, simplicity and convenience in operation, rapid reaction, accurate results, suitableness for field detection and the like, and are suitable for accurate and simple clinical detection requirements.

Description

technical field [0001] The patent of the invention relates to the technical field of medical clinical diagnosis, in particular to a rapid quantitative detection device and detection method for simultaneously detecting heart-type fatty acid binding protein and troponin I prepared by an immunocolloidal gold rapid detection method. Background technique [0002] Heart-type fatty acid binding protein (FABP) is a novel small cytosolic protein abundant in the heart. It is highly cardiac specific and is only expressed at low concentrations in tissues other than the heart. After myocardial ischemic injury, hFABP can be found in the blood as early as 1-3 hours after the onset of chest pain, peak at 6-8 hours and the plasma level returns to normal within 24-30 hours. Cardiac fatty acid binding cytoplasmic protein consists of 132 amino acids with a molecular weight of 15 kDa. The FABP gene is located on chromosome I. It is one of the most abundant proteins in the heart. The biologic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/558G01N33/532
CPCG01N33/6887G01N33/532G01N33/54386G01N33/558
Inventor 李洲杨发青周鸿锐
Owner 天津中新科炬生物制药股份有限公司
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