Method for directly planting dendrobium fimbriatum tissue culture seedlings in field

A technology of Dendrobium fringe and cultivation method, which is applied in the field of agriculture, can solve the problems of long domestication period for greenhouse hardening seedlings, insufficient supply of seedlings, and high cost, so as to improve the survival rate of transplanting and the rate of seedling yield, save the cost of cultivation, and use the effect Good results

Active Publication Date: 2014-02-05
GUIZHOU CROP VARIETIES RESOURCE INST
View PDF3 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the "bottleneck problem" of artificial cultivation of Dendrobium fringe is that the supply of seedlings is seriously insufficient. At present, there are two main methods for providing seedlings. One is the industrial production technology of tissue cultured seedlings. This technology is relatively mature. The tissue cultured seedlings reported so far The survival rate of transplanting directly out of the bottle to the wild environment for cultivation is extremely low, and it takes more than one year to domesticate the seedlings in the greenhouse. Obtaining domesticated seedlings and then transplanting them in the field can increase th...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0013] Embodiments of the present invention: the direct field cultivation method of dendrobium dendrobii tissue culture seedlings, comprises the steps:

[0014] 1) Optimize the medium, medium treatment, use 1 / 2MS medium, add potato juice accounting for 10% of the mass of 1 / 2MS medium, do not add any auxin, use this medium for tissue culture of Dendrobium fringe Rooting culture of seedlings, when the seedlings are cultivated to a plant height of more than 8cm, a stem diameter of more than 3mm, and more than 3 roots, they will be released from the bottle; after cleaning the tissue culture seedlings out of the bottle, soak them in a 1000-fold dilution of carbendazim 5min, transplant after drying;

[0015] 2) The vegetation canopy density of the cultivation environment is above 0.7, and the air humidity is above 50%; choose trees or limestone with a DBH above 10cm, and the rocks or trunks and their bases are covered with mosses;

[0016] 3) Fix the dried Dendrobium fringe tissue ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for directly planting dendrobium fimbriatum tissue culture seedlings in a field. According to the method, culture media are optimized, and after seedlings are obtained by using the optimized culture media through tissue culture, the seedlings are planted in the specific environment and by adopting a unique matrix. By adopting the method, the dendrobium fimbriatum tissue culture seedlings are directly planted in the field, and acclimation is needless, as a result, the culturing cost of the seedlings is reduced, and transplantation survival rate and seedling obtaining rate of the seedlings are remarkably improved.

Description

technical field [0001] The invention relates to the technical field of agriculture, in particular to a direct field cultivation method for tissue cultured seedlings of dendrobium fringe. Background technique [0002] my country's traditional precious Chinese medicinal materials, Guizhou authentic Chinese medicinal materials Dendrobium fringe ( Dendrobium fimbriatum ) Fresh or dried stems, used for yin deficiency, dry mouth, polydipsia, retching due to lack of food, deficiency and heat after illness, and blurred vision ("Chinese Pharmacopoeia" 2010). Dendrobium fringe is a perennial epiphyte orchid, mainly distributed in Guizhou, Guangxi, Yunnan and other places. It is suitable for epiphyton on the surface of limestone in karst mountainous areas. Southwest Guizhou is one of the best suitable areas; Dendrobium dendrobii can be used as one of the best herb species for ecological restoration of karst rocky desertification, which can make the original barren rocky desertification...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01G31/00
Inventor 吴明开刘海
Owner GUIZHOU CROP VARIETIES RESOURCE INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap