Method for preparing protein peptide by carrying out enzymolysis on marine low-value small fishes by squid visceral enzyme

A technology of squid viscera and small miscellaneous fish, which is applied in the field of protein peptide preparation to achieve the effects of simple process, pollution reduction and short production cycle

Active Publication Date: 2014-02-05
JIANGSU TIANFULAI GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no technological conditions for using squid viscera to enzymatically hydrolyze marine low-value small trash fish at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1, a method for preparing protein peptides by using squid visceral enzymes to enzymolyze low-value marine fish, the steps are as follows:

[0016] (1) Grinding and homogenizing: put fresh or frozen marine low-value small trash fish into the grinder, add water according to the weight ratio of small trash fish and water at 1:3, grind and homogenize, and obtain small trash fish homogenate;

[0017] (2) Enzymolysis: Use a pump to pump the homogenate of small miscellaneous fish into the enzymolysis tank, add squid viscera enzyme solution or squid viscera enzyme with enzyme activity ≥ 12u / mL according to the protein mass percentage of squid viscera enzyme and substrate 40% The enzymatic hydrolysis process parameters are as follows: enzymolysis time 50min, enzymolysis temperature 45°C, enzymolysis pH 7, during the enzymolysis process, use a sodium hydroxide solution with a concentration of 10 mol / L to adjust the pH. Stir to obtain the enzymolysis solution;

[0018] (...

Embodiment 2

[0021] Example 2, a method for preparing protein peptides by using squid visceral enzymes to enzymolyze low-value marine fish, the steps are as follows:

[0022] (1) Grinding and homogenizing: put fresh or frozen marine low-value small trash fish into the grinder, add water according to the weight ratio of small trash fish and water at 1:7, grind and homogenize, and obtain small trash fish homogenate;

[0023] (2) Enzymolysis: use a pump to pump the homogenate of small miscellaneous fish into the enzymolysis tank, and add squid viscera enzyme solution or squid viscera enzyme with enzyme activity ≥ 12u / mL according to the protein mass percentage of squid viscera enzyme and substrate 65% The enzymolysis process parameters are as follows: enzymolysis time 150min, enzymolysis temperature 60°C, enzymolysis pH 10, during the enzymolysis process, use a sodium hydroxide solution with a concentration of 10 mol / L to adjust the pH. Stir to obtain the enzymolysis solution;

[0024] (3) ...

Embodiment 3

[0027] Example 3, a method for preparing protein peptides by using squid visceral enzymes to enzymolyze low-value marine fish, the steps are as follows:

[0028] (1) Grinding and homogenizing: put fresh or frozen marine low-value small trash fish into the grinder, add water according to the weight ratio of small trash fish and water at 1:5, grind and homogenize, and obtain small trash fish homogenate;

[0029] (2) Enzymolysis: Use a pump to pump the homogenate of small miscellaneous fish into the enzymolysis tank, add squid viscera enzyme solution or squid viscera enzyme with enzyme activity ≥ 12u / mL according to the protein mass percentage of squid viscera enzyme and substrate 45% The enzymatic hydrolysis process parameters are as follows: enzymatic hydrolysis time 100min, enzymatic hydrolysis temperature 50°C, enzymatic hydrolysis pH 9.5, during the enzymolysis process, use a sodium hydroxide solution with a concentration of 10 mol / L to adjust the pH, during enzymolysis Sti...

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Abstract

The invention relates to a method for preparing protein peptide by carrying out enzymolysis on marine low-value small fishes by squid visceral enzyme. The method takes the marine low-value small fishes as a raw material, and comprises the steps of unfreezing, carrying out enzymolysis on the substrate small fishes by the squid visceral enzyme after homogenate treatment, and controlling the solid-to-liquid ratio, the temperature, the pH and the enzymolysis time in the enzymolysis process; after that, carrying out enzyme deactivation and concentration to obtain protein peptide paste, or carrying out spray drying treatment on the protein peptide paste to obtain protein peptide powder. The method has the advantages of being simple in technology, low in production cost, high in yield of finished products, suitable for industrial product, etc. The method not only reduces the dosage of industrial protease, but also enables the protein resource to be comprehensively utilized, avoids the environmental pollution and realizes the industrial application of the squid visceral enzyme.

Description

technical field [0001] The present invention relates to a method for preparing protein peptides, in particular to a method for preparing protein peptides by using squid visceral enzymes to enzymatically hydrolyze small marine low-value miscellaneous fish. Background technique [0002] my country is a large fishery country. During the fishing and processing process, a large number of low-value fish and aquatic product processing waste, such as internal organs, skins, eyes, and eye sockets, are discarded, causing environmental pollution. This is not only a huge waste of fishery resources, but also has the hidden danger of environmental pollution. With the improvement of people's living standards and demands, the edible value of low-value small trash fish is very low, and some of them are processed into fish meal, and protein resources are not fully utilized. Therefore, how to effectively process and utilize aquatic product processing waste and carry out high-value comprehensi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/10
Inventor 朱勤陈忆凤
Owner JIANGSU TIANFULAI GROUP
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