Filtering method for tetracycline fermentation liquor

A filtration method and fermentation liquid technology, applied in the field of biology and medicine, can solve the problems of easy degradation of tetracycline, inability to filter, high labor intensity, etc., improve labor intensity and working environment, reduce the probability of degradation reaction, and the degree of automation Excellent effect

Active Publication Date: 2015-05-13
NINGXIA QIYUAN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has defects such as large one-time equipment investment, intermittent operation, high labor intensity, many personnel, and poor working environment. Especially when dealing with low filtration rate and abnormal tank batches, it is basically helpless and unable to filter. this caused a great loss
And because the filtration, top washing process cycle is as long as 20 hours, the acidification release liquid, filtrate and lotion are under the strong acid condition of PH1.5~2.0 for a long time, tetracycline is prone to degradation reaction, causing the quality of intermediate tetracycline base to decline, Among them, the increase of 4-different to tetracycline is relatively large

Method used

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  • Filtering method for tetracycline fermentation liquor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Take 1L of tetracycline fermentation broth, measure its titer unit as 30150u / ml, add 1.6L of drinking water, dilute the fermented broth titer unit to 12000u / ml, stir well and cool down to 14°C, add 60g of oxalic acid to adjust the pH of the diluted solution =1.8, then add 5.0g of yellow blood salt, 4.0g of zinc sulfate, 1.5g of borax and other purifying agents in turn for purification treatment, and then separate with a decanter centrifuge to obtain 1.80L of separation liquid and 0.8L of slag liquid. Dilute 0.8L of slag liquid with 1.2L of oxalic acid water with pH=1.8, stir for 30 minutes at a temperature of 14°C, and separate with a decanter centrifuge to obtain 1.4L of separated liquid and 0.6L of slag liquid. Then dilute 0.6L of slag liquid with 2.4L of oxalic acid water with pH 1.8, stir for 30 minutes at a temperature of 14°C, and separate with a decanter centrifuge to obtain 2.0L of separated liquid and 1.0L of slag liquid. The slag liquid was treated repeatedly ...

Embodiment 2

[0015] Take 2L of tetracycline fermentation broth, measure its titer unit as 32080u / ml, add 2L of drinking water, dilute the fermented broth titer unit to 16000u / ml, stir well and cool down to 13°C, add 118g of oxalic acid to adjust the pH= 1.8, then add 10.0g of yellow blood salt, 9.0g of zinc sulfate, 3.0g of borax and other purifiers in turn for purification treatment, and then separate with a decanter centrifuge to obtain 2.8L of separation liquid and 1.2L of slag liquid. Dilute 1.2L of slag liquid with 2.8L of oxalic acid water with pH=1.9, control the temperature at 14°C, stir for 30 minutes, and separate with a decanter centrifuge to obtain 2.8L of separated liquid and 1.2L of slag liquid. Also dilute 1.2L of slag liquid with 2.8L of oxalic acid water with pH=1.9, control the temperature at 14°C, stir for 30 minutes, and separate with a decanter centrifuge to obtain 2.8L of separated liquid and 1.2L of slag liquid. The slag liquid was treated repeatedly in this way unti...

Embodiment 3

[0017] Take 2L of tetracycline fermentation broth, measure its titer unit as 31009u / ml, add 2L of drinking water, dilute the fermented broth titer unit to 18000u / ml, stir evenly and cool down to 13°C, add 124g of oxalic acid to adjust the pH= 1.6, then add 10.0g of yellow blood salt, 9.0g of zinc sulfate, 3.0g of borax and other purifiers in turn for purification treatment, and then separate with a decanter centrifuge to obtain 2.6L of separation liquid and 1.2L of slag liquid. Dilute 1.2L of slag liquid with 2.8L of oxalic acid water with pH=1.9, control the temperature at 14°C, stir for 30 minutes, and separate with a decanter centrifuge to obtain 2.8L of separated liquid and 1.2L of slag liquid. Also dilute 1.2L of slag liquid with 2.8L of acid water with pH=1.9, control the temperature at 14°C, stir for 30 minutes, and separate with a decanter centrifuge to obtain 2.8L of separated liquid and 1.2L of slag liquid. The slag liquid was treated repeatedly in this way until the...

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PUM

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Abstract

The invention relates to a filtering method for tetracycline fermentation liquor. The filtering method comprises steps of diluting the tetracycline fermentation liquor by adding water, then acidizing, purifying, separating by a horizontal spiral centrifugal machine so as to obtain a separating medium and liquid slag, then diluting the liquid slag by adding water, acidizing, separating by the horizontal spiral centrifugal machine so as to obtain a secondary separating medium and secondary liquid slag, repeatedly treating the liquid slag for 2-6 times till titer is less than or equal to 1000 u / ml, and combining the separating media. According to the method, the horizontal spiral centrifugal machine is used for separating solid and liquid of the fermentation liquor, and no filtering medium is used, so that the centrifugal machine is not restricted by the area and filtering speed of the filtering medium, and can process feed liquid which cannot be processed by a plate-and-frame filter press. The primary advantage of the filtering method is rapidness, therefore, the feed liquid can be processed timely, the time for storing and processing the feed liquid is greatly shortened, the probability of degradation reaction of tetracycline is effectively lowered, the quality of the product is obviously improved, little equipment is used, the occupied land is small, the work time is short, the labor intensity is effectively reduced, and the work environment is effectively improved.

Description

technical field [0001] The invention relates to the technical field of biology and medicine, in particular to a method for filtering tetracycline fermentation liquid. Background technique [0002] At present, the pretreatment and filtration of tetracycline fermentation broth adopts one-time dilution and acidification release (at a temperature below 15°C, use hydrochloric acid, sulfuric acid, oxalic acid, phosphoric acid, etc. to control the pH between 1.5 and 2.0, preferably oxalic acid), adding a purifier (yellow Blood salt, zinc sulfate, borax) after purification, enter the plate frame for filtration, and then top wash with acid water with PH=1.5-2.0. This method has defects such as large investment in one-time equipment, intermittent operation, high labor intensity, many personnel, and poor working environment. Especially when dealing with low filtration rate and abnormal tank batches, it is basically helpless and unable to filter. This caused great losses. And because ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C237/26C07C231/24
Inventor 沈卫军李学兵王良郭海燕邹学锋张志
Owner NINGXIA QIYUAN PHARMA
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