Filtering method for tetracycline fermentation liquor

Abstract

The invention relates to a filtering method for tetracycline fermentation liquor. The filtering method comprises steps of diluting the tetracycline fermentation liquor by adding water, then acidizing, purifying, separating by a horizontal spiral centrifugal machine so as to obtain a separating medium and liquid slag, then diluting the liquid slag by adding water, acidizing, separating by the horizontal spiral centrifugal machine so as to obtain a secondary separating medium and secondary liquid slag, repeatedly treating the liquid slag for 2-6 times till titer is less than or equal to 1000 u / ml, and combining the separating media. According to the method, the horizontal spiral centrifugal machine is used for separating solid and liquid of the fermentation liquor, and no filtering medium is used, so that the centrifugal machine is not restricted by the area and filtering speed of the filtering medium, and can process feed liquid which cannot be processed by a plate-and-frame filter press. The primary advantage of the filtering method is rapidness, therefore, the feed liquid can be processed timely, the time for storing and processing the feed liquid is greatly shortened, the probability of degradation reaction of tetracycline is effectively lowered, the quality of the product is obviously improved, little equipment is used, the occupied land is small, the work time is short, the labor intensity is effectively reduced, and the work environment is effectively improved.

Description

technical field [0001] The invention relates to the technical field of biology and medicine, in particular to a method for filtering tetracycline fermentation liquid. Background technique [0002] At present, the pretreatment and filtration of tetracycline fermentation broth adopts one-time dilution and acidification release (at a temperature below 15°C, use hydrochloric acid, sulfuric acid, oxalic acid, phosphoric acid, etc. to control the pH between 1.5 and 2.0, preferably oxalic acid), adding a purifier (yellow Blood salt, zinc sulfate, borax) after purification, enter the plate frame for filtration, and then top wash with acid water with PH=1.5-2.0. This method has defects such as large investment in one-time equipment, intermittent operation, high labor intensity, many personnel, and poor working environment. Especially when dealing with low filtration rate and abnormal tank batches, it is basically helpless and unable to filter. This caused great losses. And because ...

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Problems solved by technology

This method has defects such as large one-time equipment investment, intermittent operation, high labor intensity, many personnel, and poor working environment. Especially when dealing with low filtration rate and abnormal tank batches, it is basically helpless and unable to filter. this caused a great loss

And because the filtration, top washing process cycle is as long as 20 hours, the acidification release liquid, filtrate and lotion are under the strong acid condition of PH1.5~2.0 for a long time, tetracycline is prone to degradation reaction, causing the quality of intermediate tetracycline base to decline, Among them, the increase of 4-different to tetracycline is relatively large

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