Method for measuring content of polyphenols in fargesia denudate

Abstract

The invention provides a method for measuring the content of polyphenols in fargesia denudate. The method comprises three steps: drawing a standard curve by using gallic acid as a polyphenols standard substance, preparing a sample solution of the polyphenols in the fargesia denudate, and measuring the content of the polyphenols in a fargesia denudate sample. The method provided by the invention is low in detection limit, high in accuracy, good in repeatability and high in precision.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a method for determining the content of polyphenols in arrow bamboo by the Folin phenol method. Background technique [0002] Known as the "living fossil", the giant panda is a first-class protected animal in my country and a flagship species of wild animal protection in the world. With the change of the environment and thousands of years of evolution and adaptation, it has gradually transformed into a species dominated by subalpine bamboos. The staple food of herbivorous animals, the staple food bamboo is Arrow bamboo. [0003] Bamboo, the staple food of giant pandas, contains a relatively high content of polyphenols. Researchers believe that plant polyphenols can prevent animals from eating and reduce their digestibility. Therefore, the content of polyphenols in bamboo will affect the diet of giant pandas. Feed utilization and feed intake, but at present, researchers have li...

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Problems solved by technology

[0003] Bamboo, the staple food of giant pandas, contains a relatively high content of polyphenols. Researchers believe that plant polyphenols can prevent animals from eating and reduce their digestibility. Therefore, the content of polyphenols in bamboo will affect the diet of giant pandas. Feed utilization and feed intake, but at present, researchers have little research on the detection methods of polyphenols in bamboos, especially in Arrowhead bamboo, and have not yet established a detection method for polyphenols in Arrowhead bamboo

[0004] At present, there are only reports on the detection method of polyphenols content in other plants or pharmaceutical preparations, such as Chinese patent CN103076425A and Chinese patent CN102590435A disclose a kind of quality detection method of ragwort, which is determined by the folin phenol-phenol method The content of polyphenols in Ragbuk fruit, but the cell structure of different plant varieties is different, and the polyphenol content varies greatly, so there is no mutual reference for the extraction methods, extraction and detection conditions of polyphenols among different plant varieties value

Therefore, the determination method for the content of polyphenols in the staple food of giant pandas, bamboo, Arrowhead bamboo, has yet to be established.

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