Application of galangin in preparation of anti-tumour metastasis medicine

A technology of galangin and tumor metastasis, which can be used in anti-tumor drugs, drug combinations, pharmaceutical formulations, etc., can solve the problem of lack of effective drugs for inhibiting tumor metastasis, and achieve inhibiting cell adhesion, improving therapeutic effect, and inhibiting tumor metastasis. Effect

Inactive Publication Date: 2014-02-12
JIANGSU TARGET BIOMEDICINE RES INST +1
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  • Abstract
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The fundamental reason is that there is still a la

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  • Application of galangin in preparation of anti-tumour metastasis medicine
  • Application of galangin in preparation of anti-tumour metastasis medicine
  • Application of galangin in preparation of anti-tumour metastasis medicine

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Experimental program
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Embodiment

[0018] a. Effects of galangin on cell adhesion and spreading

[0019] The cell adhesion experiment was performed according to the literature (Kucik, D.F., Wu, C. 2005. Methods Mol Biol. 294:43-54). The 96-well plate was bottomed with 10 μg / ml / mL fibronectin (FN) and left overnight at 4°C. Take it out the next day, then wash twice with PBS, seal with 100 μL per well of 2% BSA DMEM medium, block at 37°C for 1 hour, and wash twice with 0.1% BSA DMEM medium. Adhesion and spreading experiments were carried out separately. After digesting and counting the B16F10 cells in the logarithmic growth phase, 4×10 5 / ml / mL, mixed with culture medium containing galangin 0, 25, 50 and 100 μM, set 5 duplicate wells for each concentration treatment group, and culture in 37°C incubator for 1 h. Take out the 96-well plate, absorb the liquid in each well (including non-adhered cells), and then wash away the non-adhered cells in the wells with PBS, fix the adhered cells with 3.7% paraformaldehyd...

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Abstract

The invention belongs to the field of natural medicines and in particular discloses application of galangin in inhibition of transcription and expression of an FAK (focal adhesion kinase) gene, inhibition of functions of FAK through phosphorylation, inhibition of cell adhesion, infiltration and migration and inhibition of tumour metastasis. The invention also relates to a galangin medicine prepared by adopting the conventional medicine preparation method, galangin can be combined with the conventional tumour treatment medicine and treatment method when being used, and treatment application is carried out by adopting the conventional oral, intravenous and intraperitoneal administration routes, so that a foundation is laid for further application of the galangin.

Description

technical field [0001] The invention belongs to the field of natural medicines, and relates to the pharmaceutical use of galangin in inhibiting cell adhesion, infiltration, migration and tumor metastasis, and the pharmaceutical use in inhibiting the transcription, expression and protein phosphorylation of focal adhesion kinase (FAK) gene. Background technique [0002] Tumor metastasis is the main cause of death of cancer patients, and 90% of cancer patients die from the metastasis of malignant tumors. The fundamental reason is that there is still a lack of effective drugs to inhibit tumor metastasis. Therefore, it is of great significance to discover drugs that inhibit cell migration and malignant tumor metastasis. [0003] Galangin is 3,5,7-trihydroxyflavone, which is a flavonoid monomer isolated from the dried rhizome of Alpinina plant of Zingiberaceae Alpinia. In addition, it also has a high content in propolis (He Rui , Wang Tao, Li Caijun, etc., 1999, Shizhen Guoyi Gu...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P35/04
Inventor 华子春张文静黄启来唐波
Owner JIANGSU TARGET BIOMEDICINE RES INST
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