Evaluation method for ELISA (enzyme-linked immunosorbent assay) detection kit of small molecule compound in food

Abstract

The invention discloses an evaluation method for an ELISA (enzyme-linked immunosorbent assay) detection kit of a small molecule compound, such as residue of a pesticide and veterinary drug, an additive and the like in food. The evaluation method is characterized by comprising the following steps: carrying out laboratory experiments through linear verification, selectivity verification, matrix suitability experiment, change resistance evaluation, inter-batch variation and stability evaluation; and carrying out laboratory collaborative experiment through accuracy verification. The core matrix suitability experiment comprises the steps: determining an appropriate matrix scope, verifying accuracy, calculating deviation, evaluating repeatability and confirming detection limit and quantitation limit. According to the evaluation method, laboratory verification and laboratory collaborative experiment are combined, and the commercialized ELISA detection kit can be accurately evaluated.

Description

technical field [0001] The invention relates to an evaluation technology of an ELISA detection kit, in particular to a quality evaluation method of an ELISA detection kit for small molecular compounds such as agricultural and veterinary drug residues and additives in food. Background technique [0002] The kit mainly exists as a rapid screening method in the analysis and detection work. At the beginning, it appeared as an alternative to the traditional classic method. Almost all kit methods need to use the traditional detection method when problems are found. The method is used as the basis for final confirmation (especially in food physicochemical and microbiological testing), so most people agree with some methodological instability of the kit method, such as false positive rate, false negative rate, quantitative precision, etc. poor degree etc., and accept this possible result error. It is precisely this kind of recognition that makes people ignore the more scientific an...

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Problems solved by technology

[0007] In view of the fact that there are not many mature evaluation system schemes available for reference at home and abroad, and the nature of the kit itself makes it impossible to directly copy the evaluation schemes and technologies of other products. At the same time, various complex food matrices will cause many interference factors , so how to accurately evaluate the evaluation of ELISA detection kits for small molecular compounds in food is quite technically difficult

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