Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Recombinant porcine interferon alpha double antibody sandwich method immune colloidal gold detection test strip and preparation method thereof

A technology for porcine interferon and test strips, which can be used in biological testing, measuring devices, material inspection products, etc., and can solve problems that hinder wide application

Active Publication Date: 2014-02-19
ANHUI JIUCHUAN BIOTECH +1
View PDF7 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no detection kit for recombinant porcine interferon α products on the market at present, which hinders its wide application. For the identification and verification of interferon alpha products

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Recombinant porcine interferon alpha double antibody sandwich method immune colloidal gold detection test strip and preparation method thereof
  • Recombinant porcine interferon alpha double antibody sandwich method immune colloidal gold detection test strip and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Preparation of purified anti-recombinant porcine interferon ɑ monoclonal antibody: intraperitoneally inject 0.5 ml of liquid paraffin into mice, and intraperitoneally inject 1×107 / ml anti-recombinant porcine interferon ɑ hybridoma cells to each mouse after the 7th day Suspension 0.5 ml, after 7-10 days, observe the degree of abdominal distention of the mice to collect ascites, centrifuge to remove the precipitate, then use octanoic acid-saturated ammonium sulfate to crudely purify, and then use DEAE anion exchange chromatography to further purify anti-recombinant porcine interferon α monoclonal Antibody, its purity can reach more than 90% by SDS-PAGE electrophoresis (so figure 1 shown), store at -80°C;

[0022] (2) Preparation of colloidal gold: 40 nm colloidal gold was prepared by trisodium citrate reduction method, the specific operation method is as follows: take 0.01% HAuCl 4 100 ml of aqueous solution, heated to boil, quickly added 1ml of 1% trisodium citrate...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a recombinant porcine interferon alpha double antibody sandwich method immune colloidal gold detection test strip and a preparation method thereof. According to an antigen-antibody reaction principle, a rabbit anti-recombinant porcine interferon alpha polyclonal antibody and a colloidal gold marked recombinant porcine interferon alpha monoclonal antibody are respectively fixed on a nitrocellulose membrane and a glass cellulose membrane to form the recombinant porcine interferon alpha double antibody sandwich method immune colloidal gold detection test strip. The recombinant porcine interferon alpha double antibody sandwich method immune colloidal gold detection test strip comprises four parts of a sample pad, a colloidal gold pad, the nitrocellulose membrane and a PVC (polyvinyl chloride) bottom board, and the glass cellulose membrane is used as the sample pad. A sensitive and rapid detection method of a recombinant porcine interferon alpha product is established, has high specificity and high stability, and is used for identification and validation of recombinant porcine interferon alpha products in labs or markets.

Description

technical field [0001] The present invention relates to the detection of recombinant porcine interferon α, specifically a recombinant porcine interferon α double-antibody sandwich method immune colloidal gold detection test strip and its preparation method, which are used for the detection of recombinant porcine interferon α products in laboratories and markets For identification and verification. Background technique [0002] The application of porcine interferon in animal husbandry production has received high attention. Porcine leukocyte interferon was the first to be used in veterinary clinics in China, because this kind of interferon has problems such as high process cost. The development of genetic engineering technology provides a new platform for mass production of porcine interferon. Our laboratory has obtained a recombinant porcine interferon α (preparation method of recombinant porcine α interferon, patent number 2008100201804) by using genetic engineering techn...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/532G01N33/6866G01N2333/56
Inventor 王明丽赵俊关鑫
Owner ANHUI JIUCHUAN BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com