Preparation method of blood glucose-reducing effective components of eremurus anisopterus and use of effective components
A technique for the effective parts of Heteropterus Heterophytum, which is applied in the field of preparation of effective parts for hypoglycemic effect of Heteroptera Heterophyllum, and can solve the problems of unreported hypoglycemic activity of Heteroptera Heterophytum
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Embodiment 1
[0016] Take 10Kg of the whole Herba Heterophytum, pulverize it, place it in a 50L extraction tank, and extract it by cold soaking at room temperature with 70% ethanol aqueous solution with a mass concentration of 6 times, each time is 24 hours, and combine the extracts, wherein the solid-to-liquid ratio is 1:15;
[0017] Place the extract in a rotary evaporator to concentrate under reduced pressure, add 10% ethanol aqueous solution to dissolve and disperse, add the pre-treated macroporous resin AB-8 column for adsorption, elute with 30% ethanol solution, and then Elute with 70% ethanol solution, collect 70% of the effluent, and concentrate to obtain 200 g of effective fractions with hypoglycemic activity.
Embodiment 2
[0019] Take 4Kg of the whole Herb Heteroptera, crush it, put it in a 50L extraction tank, and ultrasonically extract 3 times with a mass concentration of 95% ethanol aqueous solution at a temperature of 30°C, each time for 1 hour, and combine the extracts, where the solid-liquid ratio is 1:20;
[0020] Place the extract in a rotary evaporator to concentrate under reduced pressure, add 8% ethanol aqueous solution to dissolve and disperse, add pre-treated macroporous resin HPD300 column for adsorption, elute with 30% ethanol solution, and then use 70 % ethanol solution, and 70% of the effluent was collected and concentrated to obtain an effective fraction with 45 g of hypoglycemic activity.
Embodiment 3
[0022] Take 10Kg of the whole Herba Heterophytum, crush it, put it in a 50L extraction tank, extract it with absolute ethanol, heat it to the boiling point of the solvent and reflux for extraction once, each time for 4 hours, and combine the extracts, wherein the solid-to-liquid ratio is 1:10,
[0023] Place the extract in a rotary evaporator to concentrate under reduced pressure, add 5% ethanol aqueous solution to dissolve and disperse, add pre-treated macroporous resin HPD450 column for adsorption, elute with 30% ethanol solution, and then use 70 % ethanol solution, and 70% of the effluent was collected and concentrated to obtain 220 g of effective fractions with hypoglycemic activity.
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