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Method of extracting high-purity phycocyanin

A phycocyanin, high-purity technology, applied in the preparation methods of peptides, chemical instruments and methods, depsipeptides, etc., can solve the problems of difficulty in promotion and low extraction rate of phycocyanin, and achieve the effect of strong feasibility

Active Publication Date: 2014-03-05
YUNNAN BLUE DIAMOND BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems of low extraction rate of phycocyanin in the prior art and difficulty in promotion, the present invention provides a method for extracting high-purity phycocyanin, which is realized through the following technical scheme

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) After removing impurities from the algal body of fresh high-quality Arthrospira platensis, make spirulina powder according to the routine;

[0022] (2) According to the mass ratio of spirulina powder: buffer solution = 1:1, add a potassium phosphate buffer solution with a pH of 6.8 and a concentration of 0.1M to the spirulina powder in step (1), stir well, and store at -18 Freeze at 20°C for 20 hours, then thaw completely at 20°C, then send it into a high-pressure homogenizer, and perform high-pressure homogenization and wall breaking twice under a pressure of 55 MPa to obtain spirulina wall breaking liquid;

[0023] (3) Centrifuge the spirulina wall-broken solution obtained in step (2) at a speed of 5000r / min for 25 minutes, take the supernatant, and then use 2μm, 0.45μm and 0.2μm micro Filtrate through a filter membrane to obtain a crude phycocyanin extract;

[0024] (4) The phycocyanin crude extract obtained in step (3) is decolorized and purified through the co...

Embodiment 2

[0028] (1) After removing impurities from the dried high-quality Arthrospira platensis algae, make spirulina powder according to the routine;

[0029] (2) According to the mass ratio of spirulina powder: buffer solution = 1:0.8, add a potassium phosphate buffer solution with a pH of 6.0 and a concentration of 0.1M to the spirulina powder in step (1), stir well, and place in- Frozen at 10°C for 24 hours, then completely thawed at 25°C, sent to a high-pressure homogenizer, and subjected to high-pressure homogeneous wall breaking at a pressure of 40 MPa for 3 times to obtain spirulina wall breaking liquid;

[0030] (3) Centrifuge the spirulina wall-broken solution obtained in step (2) at a speed of 4000r / min for 30 minutes, then take the supernatant, and then filter the supernatant with 2μm, 0.45μm and 0.2μm microfiltration membrane to obtain a crude extract of phycocyanin after filtration;

[0031] (4) The crude phycocyanin extract obtained in step (3) is decolorized and purifi...

Embodiment 3

[0035] (1) After removing impurities from the algal body of fresh high-quality Arthrospira platensis, make spirulina powder according to the routine;

[0036] (2) According to the mass ratio of spirulina powder: buffer solution = 1:1.2, add a potassium phosphate buffer solution with a pH of 7 and a concentration of 0.1M to the spirulina powder in step (1), stir well, and place in- Frozen at 20°C for 12 hours, then completely thawed at 23°C, sent to a high-pressure homogenizer, and subjected to high-pressure homogeneous wall breaking at a pressure of 70 MPa for 5 times to obtain spirulina wall breaking liquid;

[0037](3) Centrifuge the spirulina wall-broken solution obtained in step (2) at a speed of 3500r / min for 20 minutes, take the supernatant, and then use 2μm, 0.45μm and 0.2μm micro Filtrate through a filter membrane to obtain a crude phycocyanin extract;

[0038] (4) The phycocyanin crude extract obtained in step (3) is decolorized and purified through D900 ion exchange...

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PUM

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Abstract

The invention provides a method of extracting high-purity phycocyanin. The method comprises the following steps of: adding buffer liquor into spirulina powder, uniformly stirring, freezing and unfreezing; then, carrying out high-pressure homogenizing and wall-breaking to obtain spirulina wall-broken liquor; taking liquid supernatant after centrifugal separation, respectively filtering by micro-filtration membranes with the bore diameters of 2 microns, 0.45 micron and 0.2 micron to obtain phycocyanin crude extract; passing through combined resin for discoloring and purifying, and collecting eluate, i.e., phycocyanin extract; purifying by a hydroxyapatite chromatographic column to obtain phycocyanin fine extract; finally, carrying out high-speed centrifugal spray drying after desalting and concentrating to obtain the high-purity phycocyanin. The phycocyanin can be extracted to the greatest extent by the method disclosed by the invention; moreover, the product is not polluted secondarily, extraction rate of the high-purity phycocyanin in the spirulina is over 80.0%, and product purity (A620 / A280) reaches 4.0.

Description

technical field [0001] The invention relates to a method for extracting high-purity phycocyanin, which belongs to the technical field of biological products. Background technique [0002] Phycocyanin is a dark blue powder isolated from algae such as spirulina as the main raw material. It is not only a kind of protein, but also an excellent natural food coloring, and it is also a good health food. Phycocyanin is one of the rare pigment proteins in nature. It is not only bright in color, but also a nutritious protein. Its amino acid composition is complete and its content of essential amino acids is high. [0003] At present, there are many extraction methods for phycocyanin in spirulina, but the loss of phycocyanin is too much, the extraction rate is low, it is difficult to extract a large amount, and it is easy to cause secondary pollution of the product, so it is not suitable for popularization and application. Therefore, it is necessary to develop a phycocyanin extractio...

Claims

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Application Information

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IPC IPC(8): C07K14/795C07K1/36C07K1/34C07K1/22C07K1/16
CPCC07K14/795
Inventor 熊巍刘海周许瑞珂
Owner YUNNAN BLUE DIAMOND BIOLOGICAL TECH CO LTD
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