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Alcohol-precipitated panax japonicus polysaccharide, and preparation method and application thereof

A technology of bamboo ginseng polysaccharides and bamboo ginseng, which is applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, organic active ingredients, etc., and can solve the problems of unguaranteed vaccine stability, crystallization precipitation, and insufficient effectiveness

Active Publication Date: 2014-03-05
湖北森元药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the continuous emergence of new vaccines, aluminum salt adjuvants have shown some problems, including: (1) Due to their physical and chemical properties, the colloidal state of aluminum salt vaccines is destroyed after freezing, so they cannot be freeze-dried and transported at low temperatures, But many peptide vaccines now must be stored at low temperature
(2) Due to the high salt content, there will be crystal precipitation after long-term storage, so that the stability of the prepared vaccine cannot be guaranteed
(6) Aluminum salt adjuvant releases antigenic substances slowly, and the effect is relatively weak. It is not effective enough for many soluble antigens, and the requirements for antigens are relatively high.
[0005] The new vaccines under development, such as genetic engineering vaccines and nucleic acid vaccines, have good antigen specificity and low toxicity, but poor immunogenicity, especially adjuvants are needed to enhance their immunogenicity and host's protective response to antigens

Method used

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  • Alcohol-precipitated panax japonicus polysaccharide, and preparation method and application thereof
  • Alcohol-precipitated panax japonicus polysaccharide, and preparation method and application thereof
  • Alcohol-precipitated panax japonicus polysaccharide, and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0101] Embodiment 1: Preparation of alcohol-precipitated ginseng polysaccharide of the present invention

[0102] The implementation steps of this embodiment are as follows:

[0103] A. Bamboo ginseng water extraction

[0104] Ginseng raw materials were crushed, and according to the weight ratio of bamboo joints to ethanol aqueous solution 1:14, the crushed ginseng was soaked in 85% ethanol aqueous solution for 1 hour, then heated and refluxed to extract and degrease, and the extract and The bamboo ginseng residue, the extract was discarded, according to the weight ratio of the bamboo ginseng residue to the distilled water 1:5, distilled water was added to the bamboo ginseng residue, heated and refluxed for 1.8h, and then separated, and the remaining bamboo ginseng The slag was repeatedly extracted twice with distilled water, the distilled water extracts were combined, and then concentrated under reduced pressure at a pressure of 0.006 MPa to obtain a concentrated water extra...

Embodiment 2

[0112] Embodiment 2: Preparation of alcohol-precipitated ginseng polysaccharide of the present invention

[0113] The implementation steps of this embodiment are as follows:

[0114] A. Bamboo ginseng water extraction

[0115] Crush the raw material of bamboo ginseng, according to the weight ratio of bamboo joints to ethanol aqueous solution 1:8, soak the crushed bamboo ginseng in 85% ethanol aqueous solution for 2.5 hours, then heat and reflux to extract and degrease, and separate to obtain the extract and bamboo ginseng residue, the extract was discarded, according to the weight ratio of bamboo ginseng residue to distilled water 1:8, distilled water was added to the bamboo ginseng residue, heated and refluxed for 4.2h, then separated, and the remaining bamboo The ginseng residue was repeatedly extracted 5 times with distilled water, the distilled water extracts were combined, and then concentrated under reduced pressure at a pressure of 0.008 MPa to obtain a concentrated wa...

Embodiment 3

[0123] Example 3: Preparation of alcohol-precipitated ginseng polysaccharide of the present invention

[0124] The implementation steps of this embodiment are as follows:

[0125] A. Bamboo ginseng water extraction

[0126] Crush the raw material of bamboo ginseng, according to the weight ratio of bamboo joints to ethanol aqueous solution 1:5, soak the crushed bamboo ginseng in 90% ethanol aqueous solution for 3.6 hours, then heat and reflux to extract and degrease, and separate to obtain the extract and bamboo ginseng residue, discard the extract, add distilled water to the bamboo ginseng residue according to the weight ratio of bamboo ginseng residue to distilled water 1:10, heat and reflux for extraction for 1 hour, then separate, and extract the remaining bamboo ginseng The slag was repeatedly extracted 3 times with distilled water, the distilled water extracts were combined, and then concentrated under reduced pressure at a pressure of 0.001 MPa to obtain a concentrated ...

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Abstract

The invention relates to a panax japonicus polysaccharide and a preparation method thereof. The method comprises the steps of performing aqueous extraction on the panax japonicus; removing protein; oxidizing, and performing alcohol precipitation, and the like. The panax japonicus polysaccharide has a remarkable adjuvant activity, wherein the 60 percent alcohol precipitated panax japonicus polysaccharide part has the strongest adjuvant activity, and has an intermediate viscosity-average molecular weight. Plant polysaccharides are widely distributed in the nature, and the application of the plant polysaccharides has a better advantage due to low toxicity and effectiveness, so that a novel thought and direction can be found for development of novel vaccine adjuvants.

Description

【Technical field】 [0001] The invention belongs to the technical field of vaccine adjuvants. More specifically, the present invention relates to a kind of alcohol-dried bamboo ginseng polysaccharide, also relates to the preparation method of the alcohol-dried bamboo ginseng polysaccharide, and also relates to the use of the alcohol-dried bamboo ginseng polysaccharide. 【Background technique】 [0002] Bamboo ginseng is a plant of the genus Panax ginseng in the Araliaceae family. The dried rhizome of Bamboo ginseng is in the shape of a bamboo whip. It is one of the rare and commonly used Chinese medicinal materials in China. Bamboo ginseng mostly grows in broad-leaved sparse forest shady wetlands or beside rocks and ditches above an altitude of 1000m. It has high requirements on soil conditions and is suitable for growth in neutral or slightly acidic environments. It likes thick humus layers, loose and fertile, and drainage Good sandy loam. It has a long history of folk applic...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61K39/39A61P37/04
Inventor 袁丁张长城刘朝奇王婷孙志伟周志勇崔倩倩万静枝何毓敏
Owner 湖北森元药业有限公司
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