Method for detoxification tissue culture and rapid propagation of dianthus caryophyllus

Abstract

The present invention discloses a method for detoxification tissue culture and rapid propagation of dianthus caryophyllus. The method comprises steps such as a heat treatment, explant surface disinfection, stem apex peeling, stem apex culture and the like, wherein lateral bud growing between the dianthus caryophyllus plant leaf axils is adopted as the explant, the sufficient sterile explants can be obtained through match use of ethanol and sodium hypochlorite, the explant can be subjected to direct differentiation on an induction culture medium to obtain the regeneration plant, and the regeneration rate of the plant achieves 67%, wherein the induction culture medium comprises 1 L of an MS culture medium, 25-35 g of sucrose, 5-8 g of agar, 1-3 mg of IAA, 0.5-1.5 mg of NAA, and 0.5-2 mg of ZT.

Description

technical field

[0001] The invention relates to a method for detoxifying carnation tissue culture and rapid propagation, which belongs to the field of plant tissue culture. Background technique

[0002] Carnation ( Dianthus caryophyllus ) is carnation, also known as lion head carnation, musk carnation, big flower carnation, Dutch carnation, which is a plant of Caryophyllaceae and genus Carnation. Carnations are mainly distributed in the temperate zone of Europe and Fujian, Hubei and other places in mainland China. They are native to the Mediterranean region. Carnations are the main export species of Kenya and the largest export flower species of Colombia in the Americas. They are found in Japan, South Korea, Malaysia and other countries in Asia A large number of cultivation, in Europe, countries such as Germany, Hungary, Italy, Poland, Spain, Turkey, the United Kingdom and the Netherlands have a large scale of cultivation, and it is one of the most commonly used flowers in ...